Site-specific characterization of the Asp-and Glu-ADP-ribosylated proteome

Yajie Zhang, Jianqi Wang, Ming Ding, Yonghao Yu

Research output: Contribution to journalArticle

171 Scopus citations

Abstract

Poly(ADP-ribosyl)ation is catalyzed by a family of enzymes known as PARPs. We describe a method to characterize the human aspartic acid-and glutamic acid-ADP-ribosylated proteome. We identified 1,048 ADP-ribosylation sites on 340 proteins involved in a wide array of nuclear functions; among these were many previously unknown PARP downstream targets whose ADP-ribosylation was sensitive to PARP inhibitor treatment. We also confirmed that iniparib had a negligible effect on PARP activity in intact cells.

Original languageEnglish (US)
Pages (from-to)981-984
Number of pages4
JournalNature methods
Volume10
Issue number10
DOIs
StatePublished - Oct 1 2013

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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