@article{f00da514765549ed91e8b467c53fcac6,
title = "Site-specific characterization of the Asp-and Glu-ADP-ribosylated proteome",
abstract = "Poly(ADP-ribosyl)ation is catalyzed by a family of enzymes known as PARPs. We describe a method to characterize the human aspartic acid-and glutamic acid-ADP-ribosylated proteome. We identified 1,048 ADP-ribosylation sites on 340 proteins involved in a wide array of nuclear functions; among these were many previously unknown PARP downstream targets whose ADP-ribosylation was sensitive to PARP inhibitor treatment. We also confirmed that iniparib had a negligible effect on PARP activity in intact cells.",
author = "Yajie Zhang and Jianqi Wang and Ming Ding and Yonghao Yu",
note = "Funding Information: We thank S. McKnight and H. Yu for discussions. We thank H. Yu, H. Mirzaei and S. Gygi for access to the tissue culture, mass spectrometry and computation facilities, respectively. pCMV-Sport6-GAR1 and pCMV-Sport6-DDX21 were kind gifts from L. Lum (University of Texas Southwestern Medical Center). This work was supported by grants from the UT Southwestern Medical Center Endowed Scholar Program, the Cancer Prevention and Research Institute of Texas (CPRIT R1103) and the Welch Foundation (I-1800) to Y.Y. Y.Y. is a Virginia Murchison Linthicum Scholar in Medical Research and a CPRIT Scholar in Cancer Research.",
year = "2013",
month = oct,
doi = "10.1038/nmeth.2603",
language = "English (US)",
volume = "10",
pages = "981--984",
journal = "Nature methods",
issn = "1548-7091",
publisher = "Public Library of Science",
number = "10",
}