SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells

Mark J. Kiel, Ömer H. Yilmaz, Toshihide Iwashita, Osman H. Yilmaz, Cox Terhorst, Sean J. Morrison

Research output: Contribution to journalArticle

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Abstract

To improve our ability to identify hematopoietic stem cells (HSCs) and their localization in vivo, we compared the gene expression profiles of highly purified HSCs and non-self-renewing multipotent hematopoietic progenitors (MPPs). Cell surface receptors of the SLAM family, including CD150, CD244, and CD48, were differentially expressed among functionally distinct progenitors. HSCs were highly purified as CD150+CD244-CD48- cells while MPPs were CD244+CD150-CD48- and most restricted progenitors were CD48+CD244+CD150 -. The primitiveness of hematopoietic progenitors could thus be predicted based on the combination of SLAM family members they expressed. This is the first family of receptors whose combinatorial expression precisely distinguishes stem and progenitor cells. The ability to purify HSCs based on a simple combination of SLAM receptors allowed us to identify HSCs in tissue sections. Many HSCs were associated with sinusoidal endothelium in spleen and bone marrow, though some HSCs were associated with endosteum. HSCs thus occupy multiple niches, including sinusoidal endothelium in diverse tissues.

Original languageEnglish (US)
Pages (from-to)1109-1121
Number of pages13
JournalCell
Volume121
Issue number7
DOIs
StatePublished - Jul 1 2005

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Stem Cell Niche
Hematopoietic Stem Cells
Stem cells
Aptitude
Endothelium
Stem Cells
Tissue
Signaling Lymphocytic Activation Molecule Family
Cell Surface Receptors
Transcriptome
Gene expression
Bone
Spleen
Bone Marrow

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells. / Kiel, Mark J.; Yilmaz, Ömer H.; Iwashita, Toshihide; Yilmaz, Osman H.; Terhorst, Cox; Morrison, Sean J.

In: Cell, Vol. 121, No. 7, 01.07.2005, p. 1109-1121.

Research output: Contribution to journalArticle

Kiel, Mark J. ; Yilmaz, Ömer H. ; Iwashita, Toshihide ; Yilmaz, Osman H. ; Terhorst, Cox ; Morrison, Sean J. / SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells. In: Cell. 2005 ; Vol. 121, No. 7. pp. 1109-1121.
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AU - Terhorst, Cox

AU - Morrison, Sean J.

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