Slc1a5 provides glutamine and asparagine necessary for bone development in mice

Deepika Sharma, Yilin Yu, Leyao Shen, Guo Fang Zhang, Courtney M. Karner

Research output: Contribution to journalArticlepeer-review

Abstract

Osteoblast differentiation is sequentially characterized by high rates of proliferation followed by increased protein and matrix synthesis, processes that require substantial amino acid acquisition and production. How osteoblasts obtain or maintain intracellular amino acid production is poorly understood. Here, we identify SLC1A5 as a critical amino acid transporter during bone development. Using a genetic and metabolomic approach, we show SLC1A5 acts cell autonomously to regulate protein synthesis and osteoblast differentiation. SLC1A5 provides both glutamine and asparagine which are essential for osteoblast differentiation. Mechanistically, glutamine and to a lesser extent asparagine support amino acid biosynthesis. Thus, osteoblasts depend on Slc1a5 to provide glutamine and asparagine, which are subsequently used to produce non-essential amino acids and support osteoblast differentiation and bone development.

Original languageEnglish (US)
Article numbere71595
JournaleLife
Volume10
DOIs
StatePublished - Oct 2021

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint

Dive into the research topics of 'Slc1a5 provides glutamine and asparagine necessary for bone development in mice'. Together they form a unique fingerprint.

Cite this