Sleep/Wake Behaviors in Mice during Pregnancy and Pregnancy-Associated Hypertensive Mice

Haruna Komiya, Chika Miyoshi, Kanako Iwasaki, Noriko Hotta-Hirashima, Aya Ikkyu, Satomi Kanno, Takato Honda, Masahiko Gosho, Hiromi Hamada, Toyomi Satoh, Akiyoshi Fukamizu, Hiromasa Funato, Masashi Yanagisawa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Study Objectives In humans and other mammals, sleep is altered during pregnancy. However, no studies have been conducted on sleep/wakefulness during pregnancy in mice. In this study, we examined sleep/wakefulness in female C57BL/6 mice during pregnancy. We also examined sleep/wake behaviors in an animal model of preeclampsia, pregnancy-associated hypertensive (PAH) mice, in which increased angiotensin causes hypertension. Methods Sleep/wake behaviors of female C57BL/6 and PAH mice were examined based on electroencephalogram (EEG) or electromyogram recordings before, during, and after pregnancy. To examine whether high blood pressure disrupts the integrity of the blood-brain barrier in PAH mice, Evans blue dye was injected intravenously. Angiotensin II receptor blocker (olmesartan)-administered PAH mice and female Tsukuba hypertensive mice were also examined. Results C57BL/6 mice showed a decreased total wake time and increased nonrapid eye movement (NREM) sleep time during late pregnancy. Rapid eye movement (REM) sleep time did not change during the course of pregnancy. PAH mice exhibited a general slowing of EEG during late pregnancy and subsequently returned to apparently normal sleep/wakefulness after delivery. All PAH mice exhibited multiple focal leakages of Evans blue dye in the brain. Spike-and-wave discharges were observed in 50% of PAH mice. Olmesartan-administered PAH mice did not show general slowing of EEG. Tsukuba hypertensive mice showed a normal time spent in wakefulness and NREM sleep and a decreased total REM sleep time. Conclusions This study showed pregnant-stage-specific changes in sleep/wakefulness in C57BL/6 mice. Furthermore, PAH mice may be useful as an animal model for eclampsia.

Original languageEnglish (US)
JournalSleep
Volume41
Issue number3
DOIs
StatePublished - Mar 1 2018

Fingerprint

Sleep
Pregnancy
Wakefulness
Inbred C57BL Mouse
Electroencephalography
Evans Blue
REM Sleep
Eye Movements
Coloring Agents
Animal Models
Hypertension
Eclampsia
Angiotensin Receptor Antagonists
Angiotensins
Electromyography
Pre-Eclampsia
Blood-Brain Barrier
Mammals

Keywords

  • animal models
  • blood pressure
  • hypertension
  • pregnancy

ASJC Scopus subject areas

  • Clinical Neurology
  • Physiology (medical)

Cite this

Sleep/Wake Behaviors in Mice during Pregnancy and Pregnancy-Associated Hypertensive Mice. / Komiya, Haruna; Miyoshi, Chika; Iwasaki, Kanako; Hotta-Hirashima, Noriko; Ikkyu, Aya; Kanno, Satomi; Honda, Takato; Gosho, Masahiko; Hamada, Hiromi; Satoh, Toyomi; Fukamizu, Akiyoshi; Funato, Hiromasa; Yanagisawa, Masashi.

In: Sleep, Vol. 41, No. 3, 01.03.2018.

Research output: Contribution to journalArticle

Komiya, H, Miyoshi, C, Iwasaki, K, Hotta-Hirashima, N, Ikkyu, A, Kanno, S, Honda, T, Gosho, M, Hamada, H, Satoh, T, Fukamizu, A, Funato, H & Yanagisawa, M 2018, 'Sleep/Wake Behaviors in Mice during Pregnancy and Pregnancy-Associated Hypertensive Mice', Sleep, vol. 41, no. 3. https://doi.org/10.1093/sleep/zsx209
Komiya H, Miyoshi C, Iwasaki K, Hotta-Hirashima N, Ikkyu A, Kanno S et al. Sleep/Wake Behaviors in Mice during Pregnancy and Pregnancy-Associated Hypertensive Mice. Sleep. 2018 Mar 1;41(3). https://doi.org/10.1093/sleep/zsx209
Komiya, Haruna ; Miyoshi, Chika ; Iwasaki, Kanako ; Hotta-Hirashima, Noriko ; Ikkyu, Aya ; Kanno, Satomi ; Honda, Takato ; Gosho, Masahiko ; Hamada, Hiromi ; Satoh, Toyomi ; Fukamizu, Akiyoshi ; Funato, Hiromasa ; Yanagisawa, Masashi. / Sleep/Wake Behaviors in Mice during Pregnancy and Pregnancy-Associated Hypertensive Mice. In: Sleep. 2018 ; Vol. 41, No. 3.
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abstract = "Study Objectives In humans and other mammals, sleep is altered during pregnancy. However, no studies have been conducted on sleep/wakefulness during pregnancy in mice. In this study, we examined sleep/wakefulness in female C57BL/6 mice during pregnancy. We also examined sleep/wake behaviors in an animal model of preeclampsia, pregnancy-associated hypertensive (PAH) mice, in which increased angiotensin causes hypertension. Methods Sleep/wake behaviors of female C57BL/6 and PAH mice were examined based on electroencephalogram (EEG) or electromyogram recordings before, during, and after pregnancy. To examine whether high blood pressure disrupts the integrity of the blood-brain barrier in PAH mice, Evans blue dye was injected intravenously. Angiotensin II receptor blocker (olmesartan)-administered PAH mice and female Tsukuba hypertensive mice were also examined. Results C57BL/6 mice showed a decreased total wake time and increased nonrapid eye movement (NREM) sleep time during late pregnancy. Rapid eye movement (REM) sleep time did not change during the course of pregnancy. PAH mice exhibited a general slowing of EEG during late pregnancy and subsequently returned to apparently normal sleep/wakefulness after delivery. All PAH mice exhibited multiple focal leakages of Evans blue dye in the brain. Spike-and-wave discharges were observed in 50{\%} of PAH mice. Olmesartan-administered PAH mice did not show general slowing of EEG. Tsukuba hypertensive mice showed a normal time spent in wakefulness and NREM sleep and a decreased total REM sleep time. Conclusions This study showed pregnant-stage-specific changes in sleep/wakefulness in C57BL/6 mice. Furthermore, PAH mice may be useful as an animal model for eclampsia.",
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AU - Komiya, Haruna

AU - Miyoshi, Chika

AU - Iwasaki, Kanako

AU - Hotta-Hirashima, Noriko

AU - Ikkyu, Aya

AU - Kanno, Satomi

AU - Honda, Takato

AU - Gosho, Masahiko

AU - Hamada, Hiromi

AU - Satoh, Toyomi

AU - Fukamizu, Akiyoshi

AU - Funato, Hiromasa

AU - Yanagisawa, Masashi

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N2 - Study Objectives In humans and other mammals, sleep is altered during pregnancy. However, no studies have been conducted on sleep/wakefulness during pregnancy in mice. In this study, we examined sleep/wakefulness in female C57BL/6 mice during pregnancy. We also examined sleep/wake behaviors in an animal model of preeclampsia, pregnancy-associated hypertensive (PAH) mice, in which increased angiotensin causes hypertension. Methods Sleep/wake behaviors of female C57BL/6 and PAH mice were examined based on electroencephalogram (EEG) or electromyogram recordings before, during, and after pregnancy. To examine whether high blood pressure disrupts the integrity of the blood-brain barrier in PAH mice, Evans blue dye was injected intravenously. Angiotensin II receptor blocker (olmesartan)-administered PAH mice and female Tsukuba hypertensive mice were also examined. Results C57BL/6 mice showed a decreased total wake time and increased nonrapid eye movement (NREM) sleep time during late pregnancy. Rapid eye movement (REM) sleep time did not change during the course of pregnancy. PAH mice exhibited a general slowing of EEG during late pregnancy and subsequently returned to apparently normal sleep/wakefulness after delivery. All PAH mice exhibited multiple focal leakages of Evans blue dye in the brain. Spike-and-wave discharges were observed in 50% of PAH mice. Olmesartan-administered PAH mice did not show general slowing of EEG. Tsukuba hypertensive mice showed a normal time spent in wakefulness and NREM sleep and a decreased total REM sleep time. Conclusions This study showed pregnant-stage-specific changes in sleep/wakefulness in C57BL/6 mice. Furthermore, PAH mice may be useful as an animal model for eclampsia.

AB - Study Objectives In humans and other mammals, sleep is altered during pregnancy. However, no studies have been conducted on sleep/wakefulness during pregnancy in mice. In this study, we examined sleep/wakefulness in female C57BL/6 mice during pregnancy. We also examined sleep/wake behaviors in an animal model of preeclampsia, pregnancy-associated hypertensive (PAH) mice, in which increased angiotensin causes hypertension. Methods Sleep/wake behaviors of female C57BL/6 and PAH mice were examined based on electroencephalogram (EEG) or electromyogram recordings before, during, and after pregnancy. To examine whether high blood pressure disrupts the integrity of the blood-brain barrier in PAH mice, Evans blue dye was injected intravenously. Angiotensin II receptor blocker (olmesartan)-administered PAH mice and female Tsukuba hypertensive mice were also examined. Results C57BL/6 mice showed a decreased total wake time and increased nonrapid eye movement (NREM) sleep time during late pregnancy. Rapid eye movement (REM) sleep time did not change during the course of pregnancy. PAH mice exhibited a general slowing of EEG during late pregnancy and subsequently returned to apparently normal sleep/wakefulness after delivery. All PAH mice exhibited multiple focal leakages of Evans blue dye in the brain. Spike-and-wave discharges were observed in 50% of PAH mice. Olmesartan-administered PAH mice did not show general slowing of EEG. Tsukuba hypertensive mice showed a normal time spent in wakefulness and NREM sleep and a decreased total REM sleep time. Conclusions This study showed pregnant-stage-specific changes in sleep/wakefulness in C57BL/6 mice. Furthermore, PAH mice may be useful as an animal model for eclampsia.

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