Slit2 regulates the dispersal of oligodendrocyte precursor cells via Fyn/RhoA signaling

Xiujie Liu, Yan Lu, Yong Zhang, Yuanyuan Li, Jiazhen Zhou, Yimin Yuan, Xiaofei Gao, Zhida Su, Cheng He

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Oligodendrocyte precursor cells (OPCs) are a unique type of glia that are responsible for the myelination of the central nervous system. OPC migration is important for myelin formation during central nervous system development and repair. However, the precise extracellular and intracellular mechanisms that regulate OPC migration remain elusive. Slits were reported to regulate neurodevelopmental processes such as migration, adhesion,axonguidance, and elongation through binding to roundabout receptors (Robos). However, the potential roles of Slits/Robos in oligodendrocytes remain unknown. In this study, Slit2 was found to be involved in regulating the dispersal of OPCs through the association between Robo1 and Fyn. Initially, we examined the expression of Robos in OPCs both in vitro and in vivo. Subsequently, the Boyden chamber assay showed that Slit2 could inhibit OPC migration. RoboN, a specific inhibitor of Robos, could significantly attenuate this effect. The effects were confirmed through the explant migration assay. Furthermore, treating OPCs with Slit2 protein deactivated Fyn and increased the level of activated RhoA-GTP. Finally, Fyn was found to form complexes with Robo1, but this association was decreased after Slit2 stimulation. Thus, we demonstrate for the first time that Slit2 regulates the dispersal of oligodendrocyte precursor cells through Fyn and RhoA signaling.

Original languageEnglish (US)
Pages (from-to)17503-17516
Number of pages14
JournalJournal of Biological Chemistry
Volume287
Issue number21
DOIs
StatePublished - May 18 2012

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Slit2 regulates the dispersal of oligodendrocyte precursor cells via Fyn/RhoA signaling'. Together they form a unique fingerprint.

  • Cite this

    Liu, X., Lu, Y., Zhang, Y., Li, Y., Zhou, J., Yuan, Y., Gao, X., Su, Z., & He, C. (2012). Slit2 regulates the dispersal of oligodendrocyte precursor cells via Fyn/RhoA signaling. Journal of Biological Chemistry, 287(21), 17503-17516. https://doi.org/10.1074/jbc.M111.317610