Smooth-muscle myosin heavy-chain isoform expression in bladder-outlet obstruction

M. L. Cher, B. B. Abernathy, J. D. McConnell, P. E. Zimmern, V. K. Lin

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Bladder-outlet obstruction leads to detrusor smooth-muscle hypertrophy/hyperplasia. Despite this overall increase in muscle mass, smooth-muscle contractility decreases and bladder emptying is impaired. The goal of this study was to determine whether smooth-muscle myosin heavy-chain (MHC) alterations occur in conjunction with partial obstruction of the rabbit bladder. Total MHC and MHC-isoform protein concentrations were determined by quantitative gel electrophoresis in rabbit bladders partially obstructed for 1-4 weeks. MHC gene expression was assessed by Northern and nuclease protection assays. Two MHC isoforms (SM1/SM2) were identified in the normal rabbit bladder. After 2 weeks of obstruction the ratio of SM1/SM2 changed from 0.4:0.6 to 0.5:0.5 (P < 0.01). As compared with sham-operated values, the level of MHC mRNA decreased significantly as of 1 day after obstruction. Quantitation of MHC-isoform mRNA levels revealed a nearly 3-fold increase in the SM1/SM2 ratio. In this animal model of bladder-outlet obstruction, early changes in MHC isoforms as well as an overall decrease of MHC mRNA expression were demonstrated, suggesting that obstruction induces significant alterations in myofilament gene expression.

Original languageEnglish (US)
Pages (from-to)295-300
Number of pages6
JournalWorld Journal of Urology
Volume14
Issue number5
StatePublished - 1996

Fingerprint

Smooth Muscle Myosins
Urinary Bladder Neck Obstruction
Myosin Heavy Chains
Protein Isoforms
Urinary Bladder
Rabbits
Smooth Muscle
Nuclease Protection Assays
RNA Isoforms
Gene Expression
Messenger RNA
Myofibrils
Hypertrophy
Hyperplasia
Electrophoresis
Animal Models
Gels
Muscles

ASJC Scopus subject areas

  • Urology

Cite this

Cher, M. L., Abernathy, B. B., McConnell, J. D., Zimmern, P. E., & Lin, V. K. (1996). Smooth-muscle myosin heavy-chain isoform expression in bladder-outlet obstruction. World Journal of Urology, 14(5), 295-300.

Smooth-muscle myosin heavy-chain isoform expression in bladder-outlet obstruction. / Cher, M. L.; Abernathy, B. B.; McConnell, J. D.; Zimmern, P. E.; Lin, V. K.

In: World Journal of Urology, Vol. 14, No. 5, 1996, p. 295-300.

Research output: Contribution to journalArticle

Cher, ML, Abernathy, BB, McConnell, JD, Zimmern, PE & Lin, VK 1996, 'Smooth-muscle myosin heavy-chain isoform expression in bladder-outlet obstruction', World Journal of Urology, vol. 14, no. 5, pp. 295-300.
Cher, M. L. ; Abernathy, B. B. ; McConnell, J. D. ; Zimmern, P. E. ; Lin, V. K. / Smooth-muscle myosin heavy-chain isoform expression in bladder-outlet obstruction. In: World Journal of Urology. 1996 ; Vol. 14, No. 5. pp. 295-300.
@article{653ae67cd8854641bb219153cf4e31dd,
title = "Smooth-muscle myosin heavy-chain isoform expression in bladder-outlet obstruction",
abstract = "Bladder-outlet obstruction leads to detrusor smooth-muscle hypertrophy/hyperplasia. Despite this overall increase in muscle mass, smooth-muscle contractility decreases and bladder emptying is impaired. The goal of this study was to determine whether smooth-muscle myosin heavy-chain (MHC) alterations occur in conjunction with partial obstruction of the rabbit bladder. Total MHC and MHC-isoform protein concentrations were determined by quantitative gel electrophoresis in rabbit bladders partially obstructed for 1-4 weeks. MHC gene expression was assessed by Northern and nuclease protection assays. Two MHC isoforms (SM1/SM2) were identified in the normal rabbit bladder. After 2 weeks of obstruction the ratio of SM1/SM2 changed from 0.4:0.6 to 0.5:0.5 (P < 0.01). As compared with sham-operated values, the level of MHC mRNA decreased significantly as of 1 day after obstruction. Quantitation of MHC-isoform mRNA levels revealed a nearly 3-fold increase in the SM1/SM2 ratio. In this animal model of bladder-outlet obstruction, early changes in MHC isoforms as well as an overall decrease of MHC mRNA expression were demonstrated, suggesting that obstruction induces significant alterations in myofilament gene expression.",
author = "Cher, {M. L.} and Abernathy, {B. B.} and McConnell, {J. D.} and Zimmern, {P. E.} and Lin, {V. K.}",
year = "1996",
language = "English (US)",
volume = "14",
pages = "295--300",
journal = "World Journal of Urology",
issn = "0724-4983",
publisher = "Springer Verlag",
number = "5",

}

TY - JOUR

T1 - Smooth-muscle myosin heavy-chain isoform expression in bladder-outlet obstruction

AU - Cher, M. L.

AU - Abernathy, B. B.

AU - McConnell, J. D.

AU - Zimmern, P. E.

AU - Lin, V. K.

PY - 1996

Y1 - 1996

N2 - Bladder-outlet obstruction leads to detrusor smooth-muscle hypertrophy/hyperplasia. Despite this overall increase in muscle mass, smooth-muscle contractility decreases and bladder emptying is impaired. The goal of this study was to determine whether smooth-muscle myosin heavy-chain (MHC) alterations occur in conjunction with partial obstruction of the rabbit bladder. Total MHC and MHC-isoform protein concentrations were determined by quantitative gel electrophoresis in rabbit bladders partially obstructed for 1-4 weeks. MHC gene expression was assessed by Northern and nuclease protection assays. Two MHC isoforms (SM1/SM2) were identified in the normal rabbit bladder. After 2 weeks of obstruction the ratio of SM1/SM2 changed from 0.4:0.6 to 0.5:0.5 (P < 0.01). As compared with sham-operated values, the level of MHC mRNA decreased significantly as of 1 day after obstruction. Quantitation of MHC-isoform mRNA levels revealed a nearly 3-fold increase in the SM1/SM2 ratio. In this animal model of bladder-outlet obstruction, early changes in MHC isoforms as well as an overall decrease of MHC mRNA expression were demonstrated, suggesting that obstruction induces significant alterations in myofilament gene expression.

AB - Bladder-outlet obstruction leads to detrusor smooth-muscle hypertrophy/hyperplasia. Despite this overall increase in muscle mass, smooth-muscle contractility decreases and bladder emptying is impaired. The goal of this study was to determine whether smooth-muscle myosin heavy-chain (MHC) alterations occur in conjunction with partial obstruction of the rabbit bladder. Total MHC and MHC-isoform protein concentrations were determined by quantitative gel electrophoresis in rabbit bladders partially obstructed for 1-4 weeks. MHC gene expression was assessed by Northern and nuclease protection assays. Two MHC isoforms (SM1/SM2) were identified in the normal rabbit bladder. After 2 weeks of obstruction the ratio of SM1/SM2 changed from 0.4:0.6 to 0.5:0.5 (P < 0.01). As compared with sham-operated values, the level of MHC mRNA decreased significantly as of 1 day after obstruction. Quantitation of MHC-isoform mRNA levels revealed a nearly 3-fold increase in the SM1/SM2 ratio. In this animal model of bladder-outlet obstruction, early changes in MHC isoforms as well as an overall decrease of MHC mRNA expression were demonstrated, suggesting that obstruction induces significant alterations in myofilament gene expression.

UR - http://www.scopus.com/inward/record.url?scp=0030333926&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030333926&partnerID=8YFLogxK

M3 - Article

VL - 14

SP - 295

EP - 300

JO - World Journal of Urology

JF - World Journal of Urology

SN - 0724-4983

IS - 5

ER -