Smooth muscle myosin heavy chains are developmentally regulated in the rabbit bladder

Victor K. Lin, James B. Robertson, I. Ling Lee, Philippe E. Zimmern, John D. McConnell

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Purpose: In smooth muscle (SM), myosin heavy chain (MHC) is expressed predominantly as two isoforms, SM1 and SM2, which are encoded by a single gene and expressed by alternative splicing mechanisms. Although functional differences of these isoforms are unknown, changes in SM1/SM2 ratio have been reported in various pathophysiologic conditions. We analyzed MHC composition of bladder detrusor SM from rabbits of different ages to determine whether SM1 and SM2 isoform expressions are developmentally regulated. Materials and Methods: Rabbit bladders on the -11, -4, 1, 7, 14, 21, and 90(th) days of life were analyzed for SM MHC isoform expression at protein and mRNA levels. Porous sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), S1 protection assay, and histological analysis were employed. Results: The predominant MHC isoform in fetal and neonatal bladders was SM1. In the third postnatal week, the SM1/SM2 ratio decreased from 2.3 to 1.0. A stable SM1/SM2 ratio of 0.6 was observed in the adult animal. Although expression of SM1 mRNA was 2.6-fold greater than that of SM2 in the fetus, the relative amount of SM2 mRNA increased rapidly after birth and remained the predominant isoform throughout adult life. Developmental changes in relative amounts of SM1 and SM2 protein in bladder tissues were virtually identical to those of SM1 and SM2 mRNA. SM cell growth and disappearance of primitive mesenchyme from the bladder occurred concomitantly with the MHC isoform shift. Conclusions: The parallel temporal course of MHC mRNA and protein isoform levels suggests detrusor SM MHC expression may be developmentally regulated at the mRNA level.

Original languageEnglish (US)
Pages (from-to)1376-1380
Number of pages5
JournalJournal of Urology
Volume164
Issue number4
StatePublished - 2000

Fingerprint

Smooth Muscle Myosins
Myosin Heavy Chains
Protein Isoforms
Urinary Bladder
Rabbits
Messenger RNA
RNA Isoforms
Alternative Splicing
Mesoderm
Sodium Dodecyl Sulfate
Smooth Muscle Myocytes
Smooth Muscle
Polyacrylamide Gel Electrophoresis
Proteins
Fetus
Parturition

Keywords

  • Bladder
  • Development
  • mRNA
  • Myosin heavy chain
  • Smooth muscle

ASJC Scopus subject areas

  • Urology

Cite this

Lin, V. K., Robertson, J. B., Lee, I. L., Zimmern, P. E., & McConnell, J. D. (2000). Smooth muscle myosin heavy chains are developmentally regulated in the rabbit bladder. Journal of Urology, 164(4), 1376-1380.

Smooth muscle myosin heavy chains are developmentally regulated in the rabbit bladder. / Lin, Victor K.; Robertson, James B.; Lee, I. Ling; Zimmern, Philippe E.; McConnell, John D.

In: Journal of Urology, Vol. 164, No. 4, 2000, p. 1376-1380.

Research output: Contribution to journalArticle

Lin, VK, Robertson, JB, Lee, IL, Zimmern, PE & McConnell, JD 2000, 'Smooth muscle myosin heavy chains are developmentally regulated in the rabbit bladder', Journal of Urology, vol. 164, no. 4, pp. 1376-1380.
Lin, Victor K. ; Robertson, James B. ; Lee, I. Ling ; Zimmern, Philippe E. ; McConnell, John D. / Smooth muscle myosin heavy chains are developmentally regulated in the rabbit bladder. In: Journal of Urology. 2000 ; Vol. 164, No. 4. pp. 1376-1380.
@article{6dcbb9f4e5e34a10816fba4a6bcd276d,
title = "Smooth muscle myosin heavy chains are developmentally regulated in the rabbit bladder",
abstract = "Purpose: In smooth muscle (SM), myosin heavy chain (MHC) is expressed predominantly as two isoforms, SM1 and SM2, which are encoded by a single gene and expressed by alternative splicing mechanisms. Although functional differences of these isoforms are unknown, changes in SM1/SM2 ratio have been reported in various pathophysiologic conditions. We analyzed MHC composition of bladder detrusor SM from rabbits of different ages to determine whether SM1 and SM2 isoform expressions are developmentally regulated. Materials and Methods: Rabbit bladders on the -11, -4, 1, 7, 14, 21, and 90(th) days of life were analyzed for SM MHC isoform expression at protein and mRNA levels. Porous sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), S1 protection assay, and histological analysis were employed. Results: The predominant MHC isoform in fetal and neonatal bladders was SM1. In the third postnatal week, the SM1/SM2 ratio decreased from 2.3 to 1.0. A stable SM1/SM2 ratio of 0.6 was observed in the adult animal. Although expression of SM1 mRNA was 2.6-fold greater than that of SM2 in the fetus, the relative amount of SM2 mRNA increased rapidly after birth and remained the predominant isoform throughout adult life. Developmental changes in relative amounts of SM1 and SM2 protein in bladder tissues were virtually identical to those of SM1 and SM2 mRNA. SM cell growth and disappearance of primitive mesenchyme from the bladder occurred concomitantly with the MHC isoform shift. Conclusions: The parallel temporal course of MHC mRNA and protein isoform levels suggests detrusor SM MHC expression may be developmentally regulated at the mRNA level.",
keywords = "Bladder, Development, mRNA, Myosin heavy chain, Smooth muscle",
author = "Lin, {Victor K.} and Robertson, {James B.} and Lee, {I. Ling} and Zimmern, {Philippe E.} and McConnell, {John D.}",
year = "2000",
language = "English (US)",
volume = "164",
pages = "1376--1380",
journal = "Journal of Urology",
issn = "0022-5347",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Smooth muscle myosin heavy chains are developmentally regulated in the rabbit bladder

AU - Lin, Victor K.

AU - Robertson, James B.

AU - Lee, I. Ling

AU - Zimmern, Philippe E.

AU - McConnell, John D.

PY - 2000

Y1 - 2000

N2 - Purpose: In smooth muscle (SM), myosin heavy chain (MHC) is expressed predominantly as two isoforms, SM1 and SM2, which are encoded by a single gene and expressed by alternative splicing mechanisms. Although functional differences of these isoforms are unknown, changes in SM1/SM2 ratio have been reported in various pathophysiologic conditions. We analyzed MHC composition of bladder detrusor SM from rabbits of different ages to determine whether SM1 and SM2 isoform expressions are developmentally regulated. Materials and Methods: Rabbit bladders on the -11, -4, 1, 7, 14, 21, and 90(th) days of life were analyzed for SM MHC isoform expression at protein and mRNA levels. Porous sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), S1 protection assay, and histological analysis were employed. Results: The predominant MHC isoform in fetal and neonatal bladders was SM1. In the third postnatal week, the SM1/SM2 ratio decreased from 2.3 to 1.0. A stable SM1/SM2 ratio of 0.6 was observed in the adult animal. Although expression of SM1 mRNA was 2.6-fold greater than that of SM2 in the fetus, the relative amount of SM2 mRNA increased rapidly after birth and remained the predominant isoform throughout adult life. Developmental changes in relative amounts of SM1 and SM2 protein in bladder tissues were virtually identical to those of SM1 and SM2 mRNA. SM cell growth and disappearance of primitive mesenchyme from the bladder occurred concomitantly with the MHC isoform shift. Conclusions: The parallel temporal course of MHC mRNA and protein isoform levels suggests detrusor SM MHC expression may be developmentally regulated at the mRNA level.

AB - Purpose: In smooth muscle (SM), myosin heavy chain (MHC) is expressed predominantly as two isoforms, SM1 and SM2, which are encoded by a single gene and expressed by alternative splicing mechanisms. Although functional differences of these isoforms are unknown, changes in SM1/SM2 ratio have been reported in various pathophysiologic conditions. We analyzed MHC composition of bladder detrusor SM from rabbits of different ages to determine whether SM1 and SM2 isoform expressions are developmentally regulated. Materials and Methods: Rabbit bladders on the -11, -4, 1, 7, 14, 21, and 90(th) days of life were analyzed for SM MHC isoform expression at protein and mRNA levels. Porous sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), S1 protection assay, and histological analysis were employed. Results: The predominant MHC isoform in fetal and neonatal bladders was SM1. In the third postnatal week, the SM1/SM2 ratio decreased from 2.3 to 1.0. A stable SM1/SM2 ratio of 0.6 was observed in the adult animal. Although expression of SM1 mRNA was 2.6-fold greater than that of SM2 in the fetus, the relative amount of SM2 mRNA increased rapidly after birth and remained the predominant isoform throughout adult life. Developmental changes in relative amounts of SM1 and SM2 protein in bladder tissues were virtually identical to those of SM1 and SM2 mRNA. SM cell growth and disappearance of primitive mesenchyme from the bladder occurred concomitantly with the MHC isoform shift. Conclusions: The parallel temporal course of MHC mRNA and protein isoform levels suggests detrusor SM MHC expression may be developmentally regulated at the mRNA level.

KW - Bladder

KW - Development

KW - mRNA

KW - Myosin heavy chain

KW - Smooth muscle

UR - http://www.scopus.com/inward/record.url?scp=0033813342&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033813342&partnerID=8YFLogxK

M3 - Article

C2 - 10992418

AN - SCOPUS:0033813342

VL - 164

SP - 1376

EP - 1380

JO - Journal of Urology

JF - Journal of Urology

SN - 0022-5347

IS - 4

ER -