SNARE function analyzed in synaptobrevin/VAMP knockout mice

S. Schoch, F. Deák, A. Königstorfer, M. Mozhayeva, Y. Sara, T. C. Südhof, E. T. Kavalali

Research output: Contribution to journalArticle

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Abstract

SNAREs (soluble NSF-attachment protein receptors) are generally acknowledged as central components of membrane fusion reactions, but their precise function has remained enigmatic. Competing hypotheses suggest roles for SNAREs in mediating the specificity of fusion, catalyzing fusion, or actually executing fusion. We generated knockout mice lacking synaptobrevin/VAMP 2, the vesicular SNARE protein responsible for synaptic vesicle fusion in forebrain synapses, to make use of the exquisite temporal resolution of electrophysiology in measuring fusion. In the absence of synaptobrevin 2, spontaneous synaptic vesicle fusion and fusion induced by hypertonic sucrose were decreased @O 10- fold, but fast Ca2+-triggered fusion was decreased more than 100-fold. Thus, synaptobrevin 2 may function in catalyzing fusion reactions and stabilizing fusion intermediates but is not absolutely required for synaptic fusion.

Original languageEnglish (US)
Pages (from-to)1117-1122
Number of pages6
JournalScience
Volume294
Issue number5544
DOIs
StatePublished - Nov 2 2001

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Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
Vesicle-Associated Membrane Protein 2
R-SNARE Proteins
SNARE Proteins
Knockout Mice
Synaptic Vesicles
Membrane Fusion
Electrophysiology
Prosencephalon
Synapses
Sucrose
Proteins

ASJC Scopus subject areas

  • General

Cite this

Schoch, S., Deák, F., Königstorfer, A., Mozhayeva, M., Sara, Y., Südhof, T. C., & Kavalali, E. T. (2001). SNARE function analyzed in synaptobrevin/VAMP knockout mice. Science, 294(5544), 1117-1122. https://doi.org/10.1126/science.1064335

SNARE function analyzed in synaptobrevin/VAMP knockout mice. / Schoch, S.; Deák, F.; Königstorfer, A.; Mozhayeva, M.; Sara, Y.; Südhof, T. C.; Kavalali, E. T.

In: Science, Vol. 294, No. 5544, 02.11.2001, p. 1117-1122.

Research output: Contribution to journalArticle

Schoch, S, Deák, F, Königstorfer, A, Mozhayeva, M, Sara, Y, Südhof, TC & Kavalali, ET 2001, 'SNARE function analyzed in synaptobrevin/VAMP knockout mice', Science, vol. 294, no. 5544, pp. 1117-1122. https://doi.org/10.1126/science.1064335
Schoch S, Deák F, Königstorfer A, Mozhayeva M, Sara Y, Südhof TC et al. SNARE function analyzed in synaptobrevin/VAMP knockout mice. Science. 2001 Nov 2;294(5544):1117-1122. https://doi.org/10.1126/science.1064335
Schoch, S. ; Deák, F. ; Königstorfer, A. ; Mozhayeva, M. ; Sara, Y. ; Südhof, T. C. ; Kavalali, E. T. / SNARE function analyzed in synaptobrevin/VAMP knockout mice. In: Science. 2001 ; Vol. 294, No. 5544. pp. 1117-1122.
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