SNX9 promotes metastasis by enhancing cancer cell invasion via differential regulation of RhoGTPases

Nawal Bendris, Karla C. Williams, Carlos R. Reis, Erik S. Welf, Ping Hung Chen, Bénédicte Lemmers, Michael Hahne, Hon Sing Leong, Sandra L. Schmid

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Despite current advances in cancer research, metastasis remains the leading factor in cancer-related deaths. Here we identify sorting nexin 9 (SNX9) as a new regulator of breast cancer metastasis. We detect an increase in SNX9 expression in human breast cancer metastases compared with primary tumors and demonstrate that SNX9 expression in MDA-MB-231 breast cancer cells is necessary to maintain their ability to metastasize in a chick embryo model. Conversely, SNX9 knockdown impairs this process. In vitro studies using several cancer cell lines derived from a variety of human tumors reveal a role for SNX9 in cell invasion and identify mechanisms responsible for this novel function. We show that SNX9 controls the activation of RhoA and Cdc42 GTPases and also regulates cell motility via the modulation of well-known molecules involved in metastasis, namely RhoA-ROCK and N-WASP. In addition, we find that SNX9 is required for RhoGTPase-dependent, clathrin-independent endocytosis, and in this capacity can functionally substitute to the bona fide Rho GAP, GTPase regulator associated with focal adhesion kinase (GRAF1). Taken together, our data establish novel roles for SNX9 as a multifunctional protein scaffold that regulates, and potentially coordinates, several cellular processes that together can enhance cancer cell metastasis.

Original languageEnglish (US)
Pages (from-to)1409-1419
Number of pages11
JournalMolecular Biology of the Cell
Volume27
Issue number9
DOIs
StatePublished - May 1 2016

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Sorting Nexins
Neoplasm Metastasis
Neoplasms
Breast Neoplasms
Focal Adhesion Protein-Tyrosine Kinases
rho GTP-Binding Proteins
Clathrin
GTP Phosphohydrolases
Chick Embryo
Endocytosis
Cell Movement

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

SNX9 promotes metastasis by enhancing cancer cell invasion via differential regulation of RhoGTPases. / Bendris, Nawal; Williams, Karla C.; Reis, Carlos R.; Welf, Erik S.; Chen, Ping Hung; Lemmers, Bénédicte; Hahne, Michael; Leong, Hon Sing; Schmid, Sandra L.

In: Molecular Biology of the Cell, Vol. 27, No. 9, 01.05.2016, p. 1409-1419.

Research output: Contribution to journalArticle

Bendris, Nawal ; Williams, Karla C. ; Reis, Carlos R. ; Welf, Erik S. ; Chen, Ping Hung ; Lemmers, Bénédicte ; Hahne, Michael ; Leong, Hon Sing ; Schmid, Sandra L. / SNX9 promotes metastasis by enhancing cancer cell invasion via differential regulation of RhoGTPases. In: Molecular Biology of the Cell. 2016 ; Vol. 27, No. 9. pp. 1409-1419.
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