SNX9 regulates dynamin assembly and is required for efficient clathrin-mediated endocytosis

Fabienne Soulet, Defne Yarar, Marilyn Leonard, Sandra L. Schmid

Research output: Contribution to journalArticle

145 Citations (Scopus)

Abstract

Dynamin, a central player in clathrin-mediated endocytosis, interacts with several functionally diverse SH3 domain-containing proteins. However, the role of these interactions with regard to dynamin function is poorly defined. We have investigated a recently identified protein partner of dynamin, SNX9, sorting nexin 9. SNX9 binds directly to both dynamin-1 and dynamin-2. Moreover by stimulating dynamin assembly, SNX9 stimulates dynamin's basal GTPase activity and potentiates assembly-stimulated GTPase activity on liposomes. In fixed cells, we observe that SNX9 partially localizes to clathrin-coated pits. Using total internal reflection fluorescence microscopy in living cells, we detect a transient burst of EGFP-SNX9 recruitment to clathrin-coated pits that occurs during the late stages of vesicle formation and coincides spatially and temporally with a burst of dynamin-mRFP fluorescence. Transferrin internalization is inhibited in HeLa cells after siRNA-mediated knockdown of SNX9. Thus, our results establish that SNX9 is required for efficient clathrin-mediated endocytosis and suggest that it functions to regulate dynamin activity.

Original languageEnglish (US)
Pages (from-to)2058-2067
Number of pages10
JournalMolecular Biology of the Cell
Volume16
Issue number4
DOIs
StatePublished - Apr 2005

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Dynamins
Clathrin
Endocytosis
GTP Phosphohydrolases
Sorting Nexins
Dynamin I
Dynamin II
src Homology Domains
Transferrin
HeLa Cells
Fluorescence Microscopy
Liposomes
Small Interfering RNA
Proteins
Fluorescence

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

SNX9 regulates dynamin assembly and is required for efficient clathrin-mediated endocytosis. / Soulet, Fabienne; Yarar, Defne; Leonard, Marilyn; Schmid, Sandra L.

In: Molecular Biology of the Cell, Vol. 16, No. 4, 04.2005, p. 2058-2067.

Research output: Contribution to journalArticle

Soulet, Fabienne ; Yarar, Defne ; Leonard, Marilyn ; Schmid, Sandra L. / SNX9 regulates dynamin assembly and is required for efficient clathrin-mediated endocytosis. In: Molecular Biology of the Cell. 2005 ; Vol. 16, No. 4. pp. 2058-2067.
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