Sodium barbital is a slow reversible inactivator of rabbit-muscle creatine kinase

Feng Shi, Tong Jin Zhao, Hua Wei He, Jie Li, Xian Gang Zeng, Hai Meng Zhou, Ping Wu

Research output: Contribution to journalArticle

Abstract

As a depressant of the central nervous system, the clinical effect of sodium barbital has been extensively studied. Here we report on sodium barbital as an inhibitor of rabbit-muscle creatine kinase (CK), which plays a significant role in energy homeostasis in the muscles. Although sodium barbital gradually inhibits the activity of CK with increased concentration, the inhibition effect can be completely reversed by dilution, indicating that the inactivation process is reversible. Detailed kinetics analysis, according to a previously presented theory, indicates that sodium barbital functions as a noncomplexing inhibitor, and its inhibition effect on CK is a slow reversible inactivation. In this study, a kinetic model of the substrate reaction is presented, and the microscopic rate constants for the reaction of sodium barbital with the free enzyme and the enzyme-substrate complexes are determined. Kinetic analysis reveals that sodium barbital might compete with both creatine and ATP, but mainly with creatine, to inhibit the activity of CK. The results suggest that CK might be a target for sodium barbital in vivo.

Original languageEnglish (US)
Pages (from-to)142-147
Number of pages6
JournalBiochemistry and Cell Biology
Volume84
Issue number2
DOIs
StatePublished - Apr 1 2006

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Keywords

  • Creatine kinase
  • Inactivation
  • Kinetics
  • Sodium barbital

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Shi, F., Zhao, T. J., He, H. W., Li, J., Zeng, X. G., Zhou, H. M., & Wu, P. (2006). Sodium barbital is a slow reversible inactivator of rabbit-muscle creatine kinase. Biochemistry and Cell Biology, 84(2), 142-147. https://doi.org/10.1139/o05-166