Sodium barbital is a slow reversible inactivator of rabbit-muscle creatine kinase

Feng Shi, Tong Jin Zhao, Hua Wei He, Jie Li, Xian Gang Zeng, Hai Meng Zhou, Ping Wu

Research output: Contribution to journalArticle

Abstract

As a depressant of the central nervous system, the clinical effect of sodium barbital has been extensively studied. Here we report on sodium barbital as an inhibitor of rabbit-muscle creatine kinase (CK), which plays a significant role in energy homeostasis in the muscles. Although sodium barbital gradually inhibits the activity of CK with increased concentration, the inhibition effect can be completely reversed by dilution, indicating that the inactivation process is reversible. Detailed kinetics analysis, according to a previously presented theory, indicates that sodium barbital functions as a noncomplexing inhibitor, and its inhibition effect on CK is a slow reversible inactivation. In this study, a kinetic model of the substrate reaction is presented, and the microscopic rate constants for the reaction of sodium barbital with the free enzyme and the enzyme-substrate complexes are determined. Kinetic analysis reveals that sodium barbital might compete with both creatine and ATP, but mainly with creatine, to inhibit the activity of CK. The results suggest that CK might be a target for sodium barbital in vivo.

Original languageEnglish (US)
Pages (from-to)142-147
Number of pages6
JournalBiochemistry and Cell Biology
Volume84
Issue number2
DOIs
StatePublished - Apr 2006

Fingerprint

MM Form Creatine Kinase
Barbital
Sodium
Rabbits
Creatine Kinase
Creatine
Kinetics
Central Nervous System Depressants
Substrates
Enzymes
Dilution
Muscle
Rate constants
Homeostasis
Adenosine Triphosphate
Muscles

Keywords

  • Creatine kinase
  • Inactivation
  • Kinetics
  • Sodium barbital

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Shi, F., Zhao, T. J., He, H. W., Li, J., Zeng, X. G., Zhou, H. M., & Wu, P. (2006). Sodium barbital is a slow reversible inactivator of rabbit-muscle creatine kinase. Biochemistry and Cell Biology, 84(2), 142-147. https://doi.org/10.1139/o05-166

Sodium barbital is a slow reversible inactivator of rabbit-muscle creatine kinase. / Shi, Feng; Zhao, Tong Jin; He, Hua Wei; Li, Jie; Zeng, Xian Gang; Zhou, Hai Meng; Wu, Ping.

In: Biochemistry and Cell Biology, Vol. 84, No. 2, 04.2006, p. 142-147.

Research output: Contribution to journalArticle

Shi, F, Zhao, TJ, He, HW, Li, J, Zeng, XG, Zhou, HM & Wu, P 2006, 'Sodium barbital is a slow reversible inactivator of rabbit-muscle creatine kinase', Biochemistry and Cell Biology, vol. 84, no. 2, pp. 142-147. https://doi.org/10.1139/o05-166
Shi, Feng ; Zhao, Tong Jin ; He, Hua Wei ; Li, Jie ; Zeng, Xian Gang ; Zhou, Hai Meng ; Wu, Ping. / Sodium barbital is a slow reversible inactivator of rabbit-muscle creatine kinase. In: Biochemistry and Cell Biology. 2006 ; Vol. 84, No. 2. pp. 142-147.
@article{1146665ce216484fa37b29e299ef6c0a,
title = "Sodium barbital is a slow reversible inactivator of rabbit-muscle creatine kinase",
abstract = "As a depressant of the central nervous system, the clinical effect of sodium barbital has been extensively studied. Here we report on sodium barbital as an inhibitor of rabbit-muscle creatine kinase (CK), which plays a significant role in energy homeostasis in the muscles. Although sodium barbital gradually inhibits the activity of CK with increased concentration, the inhibition effect can be completely reversed by dilution, indicating that the inactivation process is reversible. Detailed kinetics analysis, according to a previously presented theory, indicates that sodium barbital functions as a noncomplexing inhibitor, and its inhibition effect on CK is a slow reversible inactivation. In this study, a kinetic model of the substrate reaction is presented, and the microscopic rate constants for the reaction of sodium barbital with the free enzyme and the enzyme-substrate complexes are determined. Kinetic analysis reveals that sodium barbital might compete with both creatine and ATP, but mainly with creatine, to inhibit the activity of CK. The results suggest that CK might be a target for sodium barbital in vivo.",
keywords = "Creatine kinase, Inactivation, Kinetics, Sodium barbital",
author = "Feng Shi and Zhao, {Tong Jin} and He, {Hua Wei} and Jie Li and Zeng, {Xian Gang} and Zhou, {Hai Meng} and Ping Wu",
year = "2006",
month = "4",
doi = "10.1139/o05-166",
language = "English (US)",
volume = "84",
pages = "142--147",
journal = "Biochemistry and Cell Biology",
issn = "0829-8211",
publisher = "National Research Council of Canada",
number = "2",

}

TY - JOUR

T1 - Sodium barbital is a slow reversible inactivator of rabbit-muscle creatine kinase

AU - Shi, Feng

AU - Zhao, Tong Jin

AU - He, Hua Wei

AU - Li, Jie

AU - Zeng, Xian Gang

AU - Zhou, Hai Meng

AU - Wu, Ping

PY - 2006/4

Y1 - 2006/4

N2 - As a depressant of the central nervous system, the clinical effect of sodium barbital has been extensively studied. Here we report on sodium barbital as an inhibitor of rabbit-muscle creatine kinase (CK), which plays a significant role in energy homeostasis in the muscles. Although sodium barbital gradually inhibits the activity of CK with increased concentration, the inhibition effect can be completely reversed by dilution, indicating that the inactivation process is reversible. Detailed kinetics analysis, according to a previously presented theory, indicates that sodium barbital functions as a noncomplexing inhibitor, and its inhibition effect on CK is a slow reversible inactivation. In this study, a kinetic model of the substrate reaction is presented, and the microscopic rate constants for the reaction of sodium barbital with the free enzyme and the enzyme-substrate complexes are determined. Kinetic analysis reveals that sodium barbital might compete with both creatine and ATP, but mainly with creatine, to inhibit the activity of CK. The results suggest that CK might be a target for sodium barbital in vivo.

AB - As a depressant of the central nervous system, the clinical effect of sodium barbital has been extensively studied. Here we report on sodium barbital as an inhibitor of rabbit-muscle creatine kinase (CK), which plays a significant role in energy homeostasis in the muscles. Although sodium barbital gradually inhibits the activity of CK with increased concentration, the inhibition effect can be completely reversed by dilution, indicating that the inactivation process is reversible. Detailed kinetics analysis, according to a previously presented theory, indicates that sodium barbital functions as a noncomplexing inhibitor, and its inhibition effect on CK is a slow reversible inactivation. In this study, a kinetic model of the substrate reaction is presented, and the microscopic rate constants for the reaction of sodium barbital with the free enzyme and the enzyme-substrate complexes are determined. Kinetic analysis reveals that sodium barbital might compete with both creatine and ATP, but mainly with creatine, to inhibit the activity of CK. The results suggest that CK might be a target for sodium barbital in vivo.

KW - Creatine kinase

KW - Inactivation

KW - Kinetics

KW - Sodium barbital

UR - http://www.scopus.com/inward/record.url?scp=33745408160&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745408160&partnerID=8YFLogxK

U2 - 10.1139/o05-166

DO - 10.1139/o05-166

M3 - Article

VL - 84

SP - 142

EP - 147

JO - Biochemistry and Cell Biology

JF - Biochemistry and Cell Biology

SN - 0829-8211

IS - 2

ER -