Abstract
A novel analytical method for the simultaneous determination of the concentration of sildenafil and its five analogues in dietary supplements using solid-phase extraction assisted reversed-phase dispersive liquid–liquid microextraction based on solidification of floating organic droplet combined with ion-pairing liquid chromatography with an ultraviolet detector was developed. Parameters that affect extraction efficiency were systematically investigated, including the type of solid-phase extraction cartridge, pH of the extraction environment, and the type and volume of extraction and dispersive solvent. The method linearity was in the range of 5.0–100 ng/mL for sildenafil, homosildenafil, udenafil, benzylsildenafil, and thiosildenafil and 10–100 ng/mL for acetildenafil. The coefficients of determination were ≥0.996 for all regression curves. The sensitivity values expressed as limit of detection were between 2.5 and 7.5 ng/mL. Furthermore, intraday and interday precisions expressed as relative standard deviations were less than 5.7 and 9.9%, respectively. The proposed method was successfully applied to the analysis of sildenafil and its five analogues in complex dietary supplements.
Original language | English (US) |
---|---|
Pages (from-to) | 3120-3129 |
Number of pages | 10 |
Journal | Journal of Separation Science |
Volume | 40 |
Issue number | 15 |
DOIs | |
State | Published - Aug 2017 |
Keywords
- adulteration
- ion-pairing chromatography
- mixed-mode solid-phase extraction
- reversed-phase dispersive liquid-liquid microextraction
- sildenafil
ASJC Scopus subject areas
- Analytical Chemistry
- Filtration and Separation