Solution structure of the Kluyveromyces lactis LAC9 Cd2Cys6 DNA-binding domain

K. H. Gardner, S. F. Anderson, J. E. Coleman

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The Zn2Cys6 DNA-binding domain has been identified by sequence homology in approximately forty fungal proteins, including the K. lactis LAC9 transcriptional activator. Using 1H NMR spectroscopy, we have determined the solution structure of a cadmium-substituted form of the LAC9 DNA-binding domain. We have complemented this approach by applying a series of 113Cd- 1H NMR experiments, including several novel heteroTOCSY-based techniques. The DNA-binding domain forms a core of two α-helix/extended strand segments around the Cd2 binuclear cluster, with a network of amide proton-cysteinyl Sγ hydrogen bonds stabilizing the cluster. Comparison with other Zn2Cys6 domain structures provides insight into the common structural elements used in metal coordination and DNA binding.

Original languageEnglish (US)
Pages (from-to)898-905
Number of pages8
JournalNature Structural Biology
Volume2
Issue number10
StatePublished - 1995

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Kluyveromyces
DNA
Fungal Proteins
Sequence Homology
Cadmium
Amides
Nuclear magnetic resonance spectroscopy
Protons
Hydrogen
Hydrogen bonds
Magnetic Resonance Spectroscopy
Metals
Nuclear magnetic resonance
Experiments
Proton Magnetic Resonance Spectroscopy

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology

Cite this

Solution structure of the Kluyveromyces lactis LAC9 Cd2Cys6 DNA-binding domain. / Gardner, K. H.; Anderson, S. F.; Coleman, J. E.

In: Nature Structural Biology, Vol. 2, No. 10, 1995, p. 898-905.

Research output: Contribution to journalArticle

Gardner, K. H. ; Anderson, S. F. ; Coleman, J. E. / Solution structure of the Kluyveromyces lactis LAC9 Cd2Cys6 DNA-binding domain. In: Nature Structural Biology. 1995 ; Vol. 2, No. 10. pp. 898-905.
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