TY - JOUR
T1 - Solution structure of the origin DNA-binding domain of SV40 T-antigen
AU - Luo, Xuelian
AU - Sanford, David G.
AU - Bullock, Peter A.
AU - Bachovchin, William W.
PY - 1996
Y1 - 1996
N2 - The structure of the domain from simian virus 40 (SV40) large T-antigen that binds to the SV40 origin of DNA replication (T-ag-OBD131-260) has been determined by nuclear magnetic resonance spectroscopy. The overall fold, consisting of a central five-stranded antiparallel β-sheet flanked by two α-helices on one side and one α-helix and one 310-helix on the other, is a new one. Previous mutational analyses have identified two elements, termed A (-152-155) and B2 (203-207), as essential for origin-specific recognition. These elements form two closely juxtaposed loops that define a continuous surface on the protein. The addition of a duplex oligonucleotide containing the origin recognition pentanucleotide GAGGC induces chemical shift changes and slows amide proton exchange in resonances from this region, indicating that this surface directly contacts the DNA.
AB - The structure of the domain from simian virus 40 (SV40) large T-antigen that binds to the SV40 origin of DNA replication (T-ag-OBD131-260) has been determined by nuclear magnetic resonance spectroscopy. The overall fold, consisting of a central five-stranded antiparallel β-sheet flanked by two α-helices on one side and one α-helix and one 310-helix on the other, is a new one. Previous mutational analyses have identified two elements, termed A (-152-155) and B2 (203-207), as essential for origin-specific recognition. These elements form two closely juxtaposed loops that define a continuous surface on the protein. The addition of a duplex oligonucleotide containing the origin recognition pentanucleotide GAGGC induces chemical shift changes and slows amide proton exchange in resonances from this region, indicating that this surface directly contacts the DNA.
UR - http://www.scopus.com/inward/record.url?scp=0030443808&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030443808&partnerID=8YFLogxK
U2 - 10.1038/nsb1296-1034
DO - 10.1038/nsb1296-1034
M3 - Article
C2 - 8946857
AN - SCOPUS:0030443808
SN - 1072-8368
VL - 3
SP - 1034
EP - 1039
JO - Nature Structural Biology
JF - Nature Structural Biology
IS - 12
ER -