Solution structure of the RIM1α PDZ domain in complex with an ELKS1b C-terminal peptide

Jun Lu, Hongmei Li, Yun Wang, Thomas C. Südhof, Jose Rizo-Rey

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

PDZ domains are widespread protein modules that commonly recognize C-terminal sequences of target proteins and help to organize macromolecular signaling complexes. These sequences usually bind in an extended conformation to relatively shallow grooves formed between a β-strand and an α-helix in the corresponding PDZ domains. Because of this binding mode, many PDZ domains recognize primarily the C-terminal and the antepenultimate side-chains of the target protein, which commonly conform to motifs that have been categorized into different classes. However, an increasing number of PDZ domains have been found to exhibit unusual specificities. These include the PDZ domain of RIMs, which are large multidomain proteins that regulate neurotransmitter release and help to organize presynaptic active zones. The RIM PDZ domain binds to the C-terminal sequence of ELKS with a unique specificity that involves each of the four ELKS C-terminal residues. To elucidate the structural basis for this specificity, we have determined the 3D structure in solution of an RIM/ELKS C-terminal peptide complex using NMR spectroscopy. The structure shows that the RIM PDZ domain contains an unusually deep and narrow peptide-binding groove with an exquisite shape complementarity to the four ELKS C-terminal residues in their bound conformation. This groove is formed, in part, by a set of side-chains that is conserved selectively in RIM PDZ domains and that hence determines, at least in part, their unique specificity.

Original languageEnglish (US)
Pages (from-to)455-466
Number of pages12
JournalJournal of Molecular Biology
Volume352
Issue number2
DOIs
StatePublished - Sep 16 2005

Keywords

  • Active zone
  • Binding specificity
  • PDZ domain
  • Protein NMR
  • RIM

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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