Somatostatin is targeted to the regulated secretory pathway of gonadotrophs in transgenic mice expressing a metallothionein-somatostatin gene

M. J. Low, P. J. Stork, Robert E Hammer, R. L. Brinster, M. J. Warhol, G. Mandel, R. H. Goodman

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The pituitaries of transgenic mice that express a metallothionein-somatostatin fusion gene contain high concentrations of somatostatin-14 exclusively in the gonadotrophic cells. The purpose of this study was to determine whether somatostatin expressed from the foreign fusion gene enters the normal secretory pathway within these cells. Immuno-gold labeling of serial thin sections localized somatostatin to the secretory granules of gonadotropin-producing cells. The gonadotroph-specific hypophysiotropic factor, luteinizing hormone-releasing hormone caused a dose-dependent secretion of somatostatin when applied to primary pituitary cultures from these mice. Growth hormone-releasing hormone, thyrotropin-releasing hormone, corticotropin releasing factor, and dopamine did not affect somatostatin secretion. These experiments demonstrate that a neurosecretory peptide encoded by a foreign gene can enter the regulated secretory pathway of pituitary cells from transgenic mice.

Original languageEnglish (US)
Pages (from-to)16260-16263
Number of pages4
JournalJournal of Biological Chemistry
Volume261
Issue number34
StatePublished - 1986

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Gonadotrophs
Metallothionein
Secretory Pathway
Somatostatin
Transgenic Mice
Genes
Gene Fusion
Fusion reactions
Growth Hormone-Releasing Hormone
Thyrotropin-Releasing Hormone
Corticotropin-Releasing Hormone
Secretory Vesicles
Gonadotropins
Gonadotropin-Releasing Hormone
Gold
Labeling
Dopamine
Peptides

ASJC Scopus subject areas

  • Biochemistry

Cite this

Somatostatin is targeted to the regulated secretory pathway of gonadotrophs in transgenic mice expressing a metallothionein-somatostatin gene. / Low, M. J.; Stork, P. J.; Hammer, Robert E; Brinster, R. L.; Warhol, M. J.; Mandel, G.; Goodman, R. H.

In: Journal of Biological Chemistry, Vol. 261, No. 34, 1986, p. 16260-16263.

Research output: Contribution to journalArticle

Low, M. J. ; Stork, P. J. ; Hammer, Robert E ; Brinster, R. L. ; Warhol, M. J. ; Mandel, G. ; Goodman, R. H. / Somatostatin is targeted to the regulated secretory pathway of gonadotrophs in transgenic mice expressing a metallothionein-somatostatin gene. In: Journal of Biological Chemistry. 1986 ; Vol. 261, No. 34. pp. 16260-16263.
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AU - Warhol, M. J.

AU - Mandel, G.

AU - Goodman, R. H.

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