Sources of hepatic glucose production by 2H2O ingestion and Bayesian analysis of 2H glucuronide enrichment

T. C. Delgado, C. Barosa, M. M C A Castro, C. F G C Geraldes, M. Bastos, C. Baptista, A. Fagulha, L. Barros, A. Mota, M. Carvalheiro, John G. Jones, Matthew Merritt

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Abstract

The contribution of gluconeogenesis to hepatic glucose production (GP) was quantified after 2H2O ingestion by Bayesian analysis of the position 2 and 5 2H-NMR signals (H2 and H5) of monoacetone glucose (MAG) derived from urinary acetaminophen glucuronide. Six controls and 10 kidney transplant (KTx) patients with cyclosporine A (CsA) immunosuppressant therapy were studied. Seven KTx patients were lean and euglycemic (BMI = 24.3 ± 1.0 kg/m2; fasting glucose = 4.7 ± 0.1 mM) while three were obese and hyperglycemic (BMI = 30.5 ± 0.7 kg/m2; fasting glucose = 7.1 ± 0.5 mM). For the 16 spectra analyzed, the mean coefficient of variation for the gluconeogenesis contribution was 10% ± 5%. This uncertainty was associated with a mean signal-to-noise ratio (SNR) of 79:1 and 45:1 for the MAG H2 and H5 signals, respectively. For control subjects, gluconeogenesis contributed 54% ± 7% of GP as determined by the mean and standard deviation (SD) of individual Bayesian analyses. For the lean/normoglycemic KTx subjects, the gluconeogenic contribution to GP was 62% ± 7% (P = 0.06 vs. controls), while hyperglycemic/obese KTx patients had a gluconeogenic contribution of 68% ± 3% (P < 0.005 vs. controls). These data suggest that in KTx patients, an increased gluconeogenic contribution to GP is strongly associated with obesity and hyperglycemia.

Original languageEnglish (US)
Pages (from-to)517-523
Number of pages7
JournalMagnetic Resonance in Medicine
Volume60
Issue number3
DOIs
StatePublished - Sep 2008

Fingerprint

Bayes Theorem
Glucuronides
Eating
Glucose
Gluconeogenesis
Liver
Fasting
Signal-To-Noise Ratio
Immunosuppressive Agents
Hyperglycemia
Cyclosporine
Uncertainty
Obesity
Transplants
Kidney

Keywords

  • Bayesian analysis
  • Gluconeogenesis
  • Glucuronide
  • Glycogenosis
  • Posttransplant diabetes

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Delgado, T. C., Barosa, C., Castro, M. M. C. A., Geraldes, C. F. G. C., Bastos, M., Baptista, C., ... Merritt, M. (2008). Sources of hepatic glucose production by 2H2O ingestion and Bayesian analysis of 2H glucuronide enrichment. Magnetic Resonance in Medicine, 60(3), 517-523. https://doi.org/10.1002/mrm.21681

Sources of hepatic glucose production by 2H2O ingestion and Bayesian analysis of 2H glucuronide enrichment. / Delgado, T. C.; Barosa, C.; Castro, M. M C A; Geraldes, C. F G C; Bastos, M.; Baptista, C.; Fagulha, A.; Barros, L.; Mota, A.; Carvalheiro, M.; Jones, John G.; Merritt, Matthew.

In: Magnetic Resonance in Medicine, Vol. 60, No. 3, 09.2008, p. 517-523.

Research output: Contribution to journalArticle

Delgado, TC, Barosa, C, Castro, MMCA, Geraldes, CFGC, Bastos, M, Baptista, C, Fagulha, A, Barros, L, Mota, A, Carvalheiro, M, Jones, JG & Merritt, M 2008, 'Sources of hepatic glucose production by 2H2O ingestion and Bayesian analysis of 2H glucuronide enrichment', Magnetic Resonance in Medicine, vol. 60, no. 3, pp. 517-523. https://doi.org/10.1002/mrm.21681
Delgado, T. C. ; Barosa, C. ; Castro, M. M C A ; Geraldes, C. F G C ; Bastos, M. ; Baptista, C. ; Fagulha, A. ; Barros, L. ; Mota, A. ; Carvalheiro, M. ; Jones, John G. ; Merritt, Matthew. / Sources of hepatic glucose production by 2H2O ingestion and Bayesian analysis of 2H glucuronide enrichment. In: Magnetic Resonance in Medicine. 2008 ; Vol. 60, No. 3. pp. 517-523.
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