Sources of hepatic glucose production by 2H2O ingestion and Bayesian analysis of 2H glucuronide enrichment

T. C. Delgado, C. Barosa, M. M C A Castro, C. F G C Geraldes, M. Bastos, C. Baptista, A. Fagulha, L. Barros, A. Mota, M. Carvalheiro, John G. Jones, Matthew Merritt

Research output: Contribution to journalArticle

7 Scopus citations


The contribution of gluconeogenesis to hepatic glucose production (GP) was quantified after 2H2O ingestion by Bayesian analysis of the position 2 and 5 2H-NMR signals (H2 and H5) of monoacetone glucose (MAG) derived from urinary acetaminophen glucuronide. Six controls and 10 kidney transplant (KTx) patients with cyclosporine A (CsA) immunosuppressant therapy were studied. Seven KTx patients were lean and euglycemic (BMI = 24.3 ± 1.0 kg/m2; fasting glucose = 4.7 ± 0.1 mM) while three were obese and hyperglycemic (BMI = 30.5 ± 0.7 kg/m2; fasting glucose = 7.1 ± 0.5 mM). For the 16 spectra analyzed, the mean coefficient of variation for the gluconeogenesis contribution was 10% ± 5%. This uncertainty was associated with a mean signal-to-noise ratio (SNR) of 79:1 and 45:1 for the MAG H2 and H5 signals, respectively. For control subjects, gluconeogenesis contributed 54% ± 7% of GP as determined by the mean and standard deviation (SD) of individual Bayesian analyses. For the lean/normoglycemic KTx subjects, the gluconeogenic contribution to GP was 62% ± 7% (P = 0.06 vs. controls), while hyperglycemic/obese KTx patients had a gluconeogenic contribution of 68% ± 3% (P < 0.005 vs. controls). These data suggest that in KTx patients, an increased gluconeogenic contribution to GP is strongly associated with obesity and hyperglycemia.

Original languageEnglish (US)
Pages (from-to)517-523
Number of pages7
JournalMagnetic resonance in medicine
Issue number3
StatePublished - Sep 2008


  • Bayesian analysis
  • Gluconeogenesis
  • Glucuronide
  • Glycogenosis
  • Posttransplant diabetes

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'Sources of hepatic glucose production by <sup>2</sup>H<sub>2</sub>O ingestion and Bayesian analysis of <sup>2</sup>H glucuronide enrichment'. Together they form a unique fingerprint.

  • Cite this

    Delgado, T. C., Barosa, C., Castro, M. M. C. A., Geraldes, C. F. G. C., Bastos, M., Baptista, C., Fagulha, A., Barros, L., Mota, A., Carvalheiro, M., Jones, J. G., & Merritt, M. (2008). Sources of hepatic glucose production by 2H2O ingestion and Bayesian analysis of 2H glucuronide enrichment. Magnetic resonance in medicine, 60(3), 517-523.