Spatial memory formation induces recruitment of NMDA receptor and PSD-95 to synaptic lipid rafts

Ilse Delint-Ramírez, Pamela Salcedo-Tello, Federico Bermudez-Rattoni

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

NMDA receptors (NMDARs) activation in the hippocampus and insular cortex is necessary for spatial memory formation. Recent studies suggest that localization of NMDARs to lipid rafts enhance their signalization, since the kinases that phosphorylate its subunits are present in larger proportion in lipid raft membrane microdomains. We sought to determine the possibility that NMDAR translocation to synaptic lipid rafts occurs during plasticity processes such as memory formation. Our results show that water maze training induces a rapid recruitment of NMDAR subunits (NR1, NR2A, NR2B) and PSD-95 to synaptic lipid rafts and decrease in the post-synaptic density plus an increase of NR2B phosphorylation at tyrosine 1472 in the rat insular cortex. In the hippocampus, spatial training induces selective translocation of NR1 and NR2A subunits to lipid rafts. These results suggest that NMDARs translocate from the soluble fraction of post-synaptic membrane (non-raft PSD) to synaptic lipid raft during spatial memory formation. The recruitment of NMDA receptors and other proteins to lipid rafts could be an important mechanism for increasing the efficiency of synaptic transmission during synaptic plasticity process.

Original languageEnglish (US)
Pages (from-to)1658-1668
Number of pages11
JournalJournal of Neurochemistry
Volume106
Issue number4
DOIs
StatePublished - Aug 1 2008

Fingerprint

N-Methyl-D-Aspartate Receptors
Lipids
Data storage equipment
Plasticity
Cerebral Cortex
Hippocampus
Phosphorylation
Membrane Microdomains
Post-Synaptic Density
Synaptic Membranes
Neuronal Plasticity
Tyrosine
Spatial Memory
Rats
Synaptic Transmission
Phosphotransferases
Chemical activation
Membranes
Water
Efficiency

Keywords

  • Lipid raft
  • Memory
  • NMDA receptor
  • Plasticity
  • Post-synaptic density
  • Trafficking

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Spatial memory formation induces recruitment of NMDA receptor and PSD-95 to synaptic lipid rafts. / Delint-Ramírez, Ilse; Salcedo-Tello, Pamela; Bermudez-Rattoni, Federico.

In: Journal of Neurochemistry, Vol. 106, No. 4, 01.08.2008, p. 1658-1668.

Research output: Contribution to journalArticle

Delint-Ramírez, Ilse ; Salcedo-Tello, Pamela ; Bermudez-Rattoni, Federico. / Spatial memory formation induces recruitment of NMDA receptor and PSD-95 to synaptic lipid rafts. In: Journal of Neurochemistry. 2008 ; Vol. 106, No. 4. pp. 1658-1668.
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