Spatiotemporal fate map of neurogenin1 (Neurog1) lineages in the mouse central nervous system

Euiseok J. Kim, Kei Hori, Alex Wyckoff, Lauren K. Dickel, Edmund J. Koundakjian, Lisa V. Goodrich, Jane E. Johnson

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Neurog1 (Ngn1, Neurod3, neurogenin1) is a basic helix-loop-helix (bHLH) transcription factor essential for neuronal differentiation and subtype specification during embryogenesis. Due to the transient expression of Neurog1 and extensive migration of neuronal precursors, it has been challenging to understand the full complement of Neurog1 lineage cells throughout the central nervous system (CNS). Here we labeled and followed Neurog1 lineages using inducible Cre-flox recombination systems with Neurog1-Cre and Neurog1-CreERT2 BAC (bacterial artificial chromosome) transgenic mice. Neurog1 lineage cells are restricted to neuronal fates and contribute to diverse but discrete populations in each brain region. In the forebrain, Neurog1 lineages include mitral cells and glutamatergic interneurons in the olfactory bulb, pyramidal and granule neurons in the hippocampus, and pyramidal cells in the cortex. In addition, most of the thalamus, but not the hypothalamus, arises from Neurog1 progenitors. Although Neurog1 lineages are largely restricted to glutamatergic neurons, there are multiple exceptions including Purkinje cells and other GABAergic neurons in the cerebellum. This study provides the first overview of the spatiotemporal fate map of Neurog1 lineages in the CNS.

Original languageEnglish (US)
Pages (from-to)1355-1370
Number of pages16
JournalJournal of Comparative Neurology
Volume519
Issue number7
DOIs
StatePublished - May 1 2011

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Central Nervous System
Pyramidal Cells
Basic Helix-Loop-Helix Transcription Factors
Bacterial Artificial Chromosomes
GABAergic Neurons
Olfactory Bulb
Purkinje Cells
Interneurons
Prosencephalon
Thalamus
Cerebellum
Transgenic Mice
Genetic Recombination
Hypothalamus
Embryonic Development
Hippocampus
Neurons
Brain
Population

Keywords

  • BHLH transcription factor
  • Brain development
  • Genetic fate mapping
  • Neural progenitor
  • Ngn1

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Spatiotemporal fate map of neurogenin1 (Neurog1) lineages in the mouse central nervous system. / Kim, Euiseok J.; Hori, Kei; Wyckoff, Alex; Dickel, Lauren K.; Koundakjian, Edmund J.; Goodrich, Lisa V.; Johnson, Jane E.

In: Journal of Comparative Neurology, Vol. 519, No. 7, 01.05.2011, p. 1355-1370.

Research output: Contribution to journalArticle

Kim, EJ, Hori, K, Wyckoff, A, Dickel, LK, Koundakjian, EJ, Goodrich, LV & Johnson, JE 2011, 'Spatiotemporal fate map of neurogenin1 (Neurog1) lineages in the mouse central nervous system', Journal of Comparative Neurology, vol. 519, no. 7, pp. 1355-1370. https://doi.org/10.1002/cne.22574
Kim EJ, Hori K, Wyckoff A, Dickel LK, Koundakjian EJ, Goodrich LV et al. Spatiotemporal fate map of neurogenin1 (Neurog1) lineages in the mouse central nervous system. Journal of Comparative Neurology. 2011 May 1;519(7):1355-1370. https://doi.org/10.1002/cne.22574
Kim, Euiseok J. ; Hori, Kei ; Wyckoff, Alex ; Dickel, Lauren K. ; Koundakjian, Edmund J. ; Goodrich, Lisa V. ; Johnson, Jane E. / Spatiotemporal fate map of neurogenin1 (Neurog1) lineages in the mouse central nervous system. In: Journal of Comparative Neurology. 2011 ; Vol. 519, No. 7. pp. 1355-1370.
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