Spatiotemporal heterogeneity and patterning of developing renal blood vessels

Edward Daniel, D. Berfin Azizoglu, Anne R. Ryan, Tezin A. Walji, Christopher P. Chaney, Gabrielle I. Sutton, Thomas J. Carroll, Denise K. Marciano, Ondine Cleaver

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The kidney vasculature facilitates the excretion of wastes, the dissemination of hormones, and the regulation of blood chemistry. To carry out these diverse functions, the vasculature is regionalized within the kidney and along the nephron. However, when and how endothelial regionalization occurs remains unknown. Here, we examine the developing kidney vasculature to assess its 3-dimensional structure and transcriptional heterogeneity. First, we observe that endothelial cells (ECs) grow coordinately with the kidney bud as early as E10.5, and begin to show signs of specification by E13.5 when the first arteries can be identified. We then focus on how ECs pattern and remodel with respect to the developing nephron and collecting duct epithelia. ECs circumscribe nephron progenitor populations at the distal tips of the ureteric bud (UB) tree and form stereotyped cruciform structures around each tip. Beginning at the renal vesicle (RV) stage, ECs form a continuous plexus around developing nephrons. The endothelial plexus envelops and elaborates with the maturing nephron, becoming preferentially enriched along the early distal tubule. Lastly, we perform transcriptional and immunofluorescent screens to characterize spatiotemporal heterogeneity in the kidney vasculature and identify novel regionally enriched genes. A better understanding of development of the kidney vasculature will help instruct engineering of properly vascularized ex vivo kidneys and evaluate diseased kidneys.

Original languageEnglish (US)
Pages (from-to)1-18
Number of pages18
JournalAngiogenesis
DOIs
StateAccepted/In press - Apr 7 2018

Fingerprint

Endothelial cells
Blood vessels
Blood Vessels
Nephrons
Kidney
Endothelial Cells
Ducts
Blood
Genes
Hormones
Specifications
Kidney Diseases
Epithelium
Arteries
Population

Keywords

  • Blood vessel
  • Endothelial cell heterogeneity
  • Endothelium
  • Epithelium
  • Nephron
  • Vascular patterning

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cancer Research

Cite this

Daniel, E., Azizoglu, D. B., Ryan, A. R., Walji, T. A., Chaney, C. P., Sutton, G. I., ... Cleaver, O. (Accepted/In press). Spatiotemporal heterogeneity and patterning of developing renal blood vessels. Angiogenesis, 1-18. https://doi.org/10.1007/s10456-018-9612-y

Spatiotemporal heterogeneity and patterning of developing renal blood vessels. / Daniel, Edward; Azizoglu, D. Berfin; Ryan, Anne R.; Walji, Tezin A.; Chaney, Christopher P.; Sutton, Gabrielle I.; Carroll, Thomas J.; Marciano, Denise K.; Cleaver, Ondine.

In: Angiogenesis, 07.04.2018, p. 1-18.

Research output: Contribution to journalArticle

Daniel E, Azizoglu DB, Ryan AR, Walji TA, Chaney CP, Sutton GI et al. Spatiotemporal heterogeneity and patterning of developing renal blood vessels. Angiogenesis. 2018 Apr 7;1-18. https://doi.org/10.1007/s10456-018-9612-y
Daniel, Edward ; Azizoglu, D. Berfin ; Ryan, Anne R. ; Walji, Tezin A. ; Chaney, Christopher P. ; Sutton, Gabrielle I. ; Carroll, Thomas J. ; Marciano, Denise K. ; Cleaver, Ondine. / Spatiotemporal heterogeneity and patterning of developing renal blood vessels. In: Angiogenesis. 2018 ; pp. 1-18.
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