Speciation in Cloudless Sulphurs Gleaned from Complete Genomes

Qian Cong, Jinhui Shen, Andrew D. Warren, Dominika Borek, Zbyszek Otwinowski, Nick V. Grishin

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

For 200 years, zoologists have relied on phenotypes to learn about the evolution of animals. A glance at the genotype, even through several gene markers, revolutionized our understanding of animal phylogeny. Recent advances in sequencing techniques allow researchers to study speciation mechanisms and the link between genotype and phenotype using complete genomes. We sequenced and assembled a complete genome of the Cloudless Sulphur (Phoebis sennae) from a single wild-caught specimen. This genome was used as reference to compare genomes of six specimens, three from the eastern populations (Oklahoma and north Texas), referred to as a subspeciesPhoebis sennae eubule, and three from the southwestern populations (south Texas) known as a subspeciesPhoebis sennae marcellina While the two subspecies differ only subtly in phenotype and mitochondrial DNA, comparison of their complete genomes revealed consistent and significant differences, which are more prominent than those between tiger swallowtailsPterourus canadensisandPterourus glaucus The two sulphur taxa differed in histone methylation regulators, chromatin-associated proteins, circadian clock, and early development proteins. Despite being well separated on the whole-genome level, the two taxa show introgression, with gene flow mainly fromP. s. marcellinatoP. s. eubule Functional analysis of introgressed genes reveals enrichment in transmembrane transporters. Many transporters are responsible for nutrient uptake, and their introgression may be of selective advantage for caterpillars to feed on more diverse food resources. Phylogenetically, complete genomes place family Pieridae away from Papilionidae, which is consistent with previous analyses based on several gene markers.

Original languageEnglish (US)
Pages (from-to)915-931
Number of pages17
JournalGenome Biology and Evolution
Volume8
Issue number3
DOIs
StatePublished - Mar 30 2016

Keywords

  • comparative genomics
  • introgression
  • lepidoptera
  • Phoebis sennae
  • phylogeny
  • speciation

ASJC Scopus subject areas

  • Medicine(all)
  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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