Species specificity in ribosome biogenesis

A nonconserved phosphoprotein is required for formation of the large ribosomal subunit in Trypanosoma brucei

Bryan C. Jensen, Deirdre L. Brekken, Amber C. Randall, Charles T. Kifer, Marilyn Parsons

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

In the protozoan parasite Trypanosoma brucei, the large rRNA, which is a single 3.4- to 5-kb species in most organisms, is further processed to form six distinct RNAs, two larger than 1 kb (LSU1 and LSU2) and four smaller than 220 bp. The small rRNA SR1 separates the two large RNAs, while the remaining small RNAs are clustered at the 3′ end of the precursor rRNA. One would predict that T. brucei possesses specific components to carry out these added processing events. We show here that the trypanosomatid-specific nucleolar phosphoprotein NOPP44/46 is involved in this further processing. Cells depleted of NOPP44/46 by RNA interference had a severe growth defect and demonstrated a defect in large-ribosomal-subunit biogenesis. Concurrent with this defect, a significant decrease in processing intermediates, particularly for SR1, was seen. In addition, we saw an accumulation of aberrant processing intermediates caused by cleavage within either LSU1 or LSU2. Though it is required for large-subunit biogenesis, we show that NOPP44/46 is not incorporated into the nascent particle. Thus, NOPP44/46 is an unusual protein in that it is both nonconserved and required for ribosome biogenesis.

Original languageEnglish (US)
Pages (from-to)30-35
Number of pages6
JournalEukaryotic Cell
Volume4
Issue number1
DOIs
StatePublished - Jan 2005

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Large Ribosome Subunits
Species Specificity
Trypanosoma brucei
Trypanosoma brucei brucei
phosphoproteins
Phosphoproteins
ribosomes
Ribosomes
RNA
ribosomal RNA
Trypanosomatidae
RNA Precursors
RNA Interference
RNA interference
Protozoa
Parasites
parasites
organisms
Growth
biogenesis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Microbiology

Cite this

Species specificity in ribosome biogenesis : A nonconserved phosphoprotein is required for formation of the large ribosomal subunit in Trypanosoma brucei. / Jensen, Bryan C.; Brekken, Deirdre L.; Randall, Amber C.; Kifer, Charles T.; Parsons, Marilyn.

In: Eukaryotic Cell, Vol. 4, No. 1, 01.2005, p. 30-35.

Research output: Contribution to journalArticle

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