Specific association of human telomerase activity with immortal cells and cancer

Nam W. Kim, Mieczyslaw A. Piatyszek, Karen R. Prowse, Calvin B. Harley, Michael D. West, Peter L C Ho, Gina M. Coviello, Woodring E. Wright, Scott L. Weinrich, Jerry W. Shay

Research output: Contribution to journalArticle

5939 Citations (Scopus)

Abstract

Synthesis of DNA at chromosome ends by telomerase may be necessary for indefinite proliferation of human cells. A highly sensitive assay for measuring telomerase activity was developed. In cultured cells representing 18 different human tissues, 98 of 100 immortal and none of 22 mortal populations were positive for telomerase. Similarly, 90 of 101 biopsies representing 12 human tumor types and none of 50 normal somatic tissues were positive. Normal ovaries and testes were positive, but benign tumors such as fibroids were negative. Thus, telomerase appears to be stringently repressed in normal human somatic tissues but reactivated in cancer, where immortal cells are likely required to maintain tumor growth.

Original languageEnglish (US)
Pages (from-to)2011-2015
Number of pages5
JournalScience
Volume266
Issue number5193
StatePublished - 1994

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Telomerase
Human Activities
Neoplasms
Leiomyoma
Testis
Ovary
Cultured Cells
Chromosomes
Cell Proliferation
Biopsy
DNA
Growth
Population

ASJC Scopus subject areas

  • General

Cite this

Kim, N. W., Piatyszek, M. A., Prowse, K. R., Harley, C. B., West, M. D., Ho, P. L. C., ... Shay, J. W. (1994). Specific association of human telomerase activity with immortal cells and cancer. Science, 266(5193), 2011-2015.

Specific association of human telomerase activity with immortal cells and cancer. / Kim, Nam W.; Piatyszek, Mieczyslaw A.; Prowse, Karen R.; Harley, Calvin B.; West, Michael D.; Ho, Peter L C; Coviello, Gina M.; Wright, Woodring E.; Weinrich, Scott L.; Shay, Jerry W.

In: Science, Vol. 266, No. 5193, 1994, p. 2011-2015.

Research output: Contribution to journalArticle

Kim, NW, Piatyszek, MA, Prowse, KR, Harley, CB, West, MD, Ho, PLC, Coviello, GM, Wright, WE, Weinrich, SL & Shay, JW 1994, 'Specific association of human telomerase activity with immortal cells and cancer', Science, vol. 266, no. 5193, pp. 2011-2015.
Kim NW, Piatyszek MA, Prowse KR, Harley CB, West MD, Ho PLC et al. Specific association of human telomerase activity with immortal cells and cancer. Science. 1994;266(5193):2011-2015.
Kim, Nam W. ; Piatyszek, Mieczyslaw A. ; Prowse, Karen R. ; Harley, Calvin B. ; West, Michael D. ; Ho, Peter L C ; Coviello, Gina M. ; Wright, Woodring E. ; Weinrich, Scott L. ; Shay, Jerry W. / Specific association of human telomerase activity with immortal cells and cancer. In: Science. 1994 ; Vol. 266, No. 5193. pp. 2011-2015.
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