Specific cleavage of model recombination and repair intermediates by the yeast Rad1-Rad10 DNA endonuclease

A. Jane Bardwell, Lee Bardwell, Alan E. Tomkinson, Errol C. Friedberg

Research output: Contribution to journalArticle

215 Scopus citations

Abstract

The RAD1 and RAD10 genes of Saccharomyces cerevisiae are required for both nucleotide excision repair and certain mitotic recombination events. Here, model recombination and repair intermediates were used to show that Rad1-Rad10-mediated cleavage occurs at duplex-single-strand junctions. Moreover, cleavage occurs only on the strand containing the 3' single-stranded tail. Thus, both biochemical and genetic evidence indicate a role for the Rad1-Rad10 complex in the cleavage of specific recombination intermediates. Furthermore, these data suggest that Rad1-Rad10 endonuclease incises DNA 5' to damaged bases during nucleotide excision repair.

Original languageEnglish (US)
Pages (from-to)2082-2085
Number of pages4
JournalScience
Volume265
Issue number5181
DOIs
StatePublished - Sep 30 1994

ASJC Scopus subject areas

  • General

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