Specific growth response of ras-transformed embryo fibroblasts to tumour promoters

Gian Paolo Dotto, Luis F. Parada, Robert A. Weinberg

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

Chemical carcinogenesis is a process involving multiple steps, as shown in several in vivo experimental systems1. Two early steps have been well characterized: initiation, achieved by a single, subthreshold dose of a carcinogen, and promotion, induced by repetitive treatments with a non-carcinogenic tumour promoter. At the cellular level, establishment of the transformed phenotype is also a multi-step process and activation of several, independent genes appears to be required2-4. Here we show that, like initiated cells, primary rat embryo fibroblasts (REFs) containing a ras but not a myc oncogene, are strongly and specifically stimulated to grow by tumour promoters. In the presence of these promoters, ras-containing REFs acquire the ability to overgrow normal cells in the monolayer and to form foci with 100% efficiency. Similar to the in vivo situation, promoter effects can be blocked by the concomitant application of retinoic acid.

Original languageEnglish (US)
Pages (from-to)472-475
Number of pages4
JournalNature
Volume318
Issue number6045
DOIs
StatePublished - 1985

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Carcinogens
Embryonic Structures
Fibroblasts
Growth
myc Genes
Tretinoin
Carcinogenesis
Phenotype
Genes

ASJC Scopus subject areas

  • General

Cite this

Paolo Dotto, G., Parada, L. F., & Weinberg, R. A. (1985). Specific growth response of ras-transformed embryo fibroblasts to tumour promoters. Nature, 318(6045), 472-475. https://doi.org/10.1038/318472a0

Specific growth response of ras-transformed embryo fibroblasts to tumour promoters. / Paolo Dotto, Gian; Parada, Luis F.; Weinberg, Robert A.

In: Nature, Vol. 318, No. 6045, 1985, p. 472-475.

Research output: Contribution to journalArticle

Paolo Dotto, G, Parada, LF & Weinberg, RA 1985, 'Specific growth response of ras-transformed embryo fibroblasts to tumour promoters', Nature, vol. 318, no. 6045, pp. 472-475. https://doi.org/10.1038/318472a0
Paolo Dotto, Gian ; Parada, Luis F. ; Weinberg, Robert A. / Specific growth response of ras-transformed embryo fibroblasts to tumour promoters. In: Nature. 1985 ; Vol. 318, No. 6045. pp. 472-475.
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