Specific inhibition of transforming growth factor-β2 expression in human osteoblast cells by antisense phosphorothioate oligonucleotides

Zhong Jian Shen, Sang Kook Kim, Oh Shin Kwon, Young Sup Lee, Byung Jo Moon

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

To elucidate the role of endogenous transforming growth factor (TGF)-β2 on human osteoblast cell, antisense phosphorothioate oligonucleotides (S-ODNs) complementary to regions in mRNA of TGF-β2 were synthesized and examined their effects on TGF-β2 production and cell proliferation in a human osteoblast cell line ROS 17/2. Antisense S-ODNs were designated for three different target regions in the mRNA of TGF-β2. Among several antisense S-ODN analyzed, an oligonucleotide (AS-11) complementary to the translation initiation site of mRNA of TGF-β2 demonstrated a selective and strong inhibitory effect on TGF-β2 production in osteoblast cells. Other antisense S-ODNs which were designated for other regions in mRNA of TGF-β2 and one- or three-base mismatched analogs of AS-11 showed little or much less antisense activities than AS-11. Therefore, the most effective target site in mRNA of TGF-β2 is at the initiation codon region. The antisense effects of AS-11 were observed without reduction of levels of mRNA of TGF-β2. Furthermore, the inhibition of TGF-β2 expression by antisense S-ODN appeared to enhance cell proliferation, demonstrating the growth inhibitory effect of autocrine TGF-β2 in osteoblast cells.

Original languageEnglish (US)
Pages (from-to)2331-2337
Number of pages7
JournalEuropean Journal of Biochemistry
Volume268
Issue number8
DOIs
StatePublished - 2001

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Phosphorothioate Oligonucleotides
Antisense Oligonucleotides
Osteoblasts
Transforming Growth Factors
Messenger RNA
Cell proliferation
Cell Proliferation
Initiator Codon
Oligonucleotides

Keywords

  • Antisense oligonucleotides
  • Osteoblast cell
  • TGF-β2 expression

ASJC Scopus subject areas

  • Biochemistry

Cite this

Specific inhibition of transforming growth factor-β2 expression in human osteoblast cells by antisense phosphorothioate oligonucleotides. / Shen, Zhong Jian; Kim, Sang Kook; Kwon, Oh Shin; Lee, Young Sup; Moon, Byung Jo.

In: European Journal of Biochemistry, Vol. 268, No. 8, 2001, p. 2331-2337.

Research output: Contribution to journalArticle

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