Specific interaction of the PDZ domain protein PICK1 with the COOH terminus of protein kinase C-α

Jeff Staudinger, Jianrong Lu, Eric N. Olson

Research output: Contribution to journalArticle

194 Citations (Scopus)

Abstract

PICK1 is a protein kinase C (PKC) α-binding protein initially identified using the yeast two-hybrid system. Here we report that PICK1 contains a PDZ domain that interacts specifically with a previously unidentified PDZ-binding domain (QSAV) at the extreme COOH terminus of PKCα and that mutation of a putative carboxylate-binding loop within the PICK1 PDZ domain abolishes this interaction. The PDZ-binding domain in PKCα is absent from other PKC isoforms that do not interact with PICK1. We also demonstrate that PICK1 can homooligomerize through sequences that are distinct from the carboxylate-binding loop, suggesting that self-association and PKCα binding are not mutually exclusive. A Caenorhabditis elegans PICK1-like protein is also able to bind to PKCα, suggesting a conservation of function through evolution. Association of PKCα with PICK1 provides a potential mechanism for the selective targeting of PKCα to unique subcellular sites.

Original languageEnglish (US)
Pages (from-to)32019-32024
Number of pages6
JournalJournal of Biological Chemistry
Volume272
Issue number51
DOIs
StatePublished - Dec 19 1997

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PDZ Domains
Protein Kinase C
Proteins
Two-Hybrid System Techniques
Caenorhabditis elegans
Hybrid systems
Yeast
Conservation
Carrier Proteins
Protein Isoforms
Mutation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Specific interaction of the PDZ domain protein PICK1 with the COOH terminus of protein kinase C-α. / Staudinger, Jeff; Lu, Jianrong; Olson, Eric N.

In: Journal of Biological Chemistry, Vol. 272, No. 51, 19.12.1997, p. 32019-32024.

Research output: Contribution to journalArticle

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