Specific regional distribution of gec1 mRNAs in adult rat central nervous system

Fabrice Tolle, Pierre Yves Risold, Virginie Mansuy-Schlick, Emilie Rossi, Michaël Boyer-Guittaut, Annick Fraichard, Michèle Jouvenot

Research output: Contribution to journalArticle

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Abstract

GEC1 protein shares high identity with GABARAP (GABAA Receptor-Associated Protein), interacts with tubulin and GABAA receptors and is potentially involved in intracellular transport processes. Recently, using quantitative real time PCR, we have reported the gec1 mRNA expression in different rat brain areas. In the present study, we investigated the cell types expressing gec1 in rat brain. Sense and anti-sense gec1 RNA probes, corresponding to the 3′-untranslated region, were generated. In northern blotting experiments, the anti-sense probe revealed only the 1.75 kb gec1 mRNAs. On the other hand, in immunohistochemistry experiments, GEC1 polyclonal antibodies did not discriminate between GEC1 and GABARAP proteins. Therefore, we used digoxigenin-labeled RNA probes for in situ hybridization (ISH) experiments to map the gec1 expression. Using the anti-sense probe, we detected the gec1 mRNAs specifically in neurons throughout the rostrocaudal extent of the brain as well as in the spinal cord. Although a majority of neurons expressed the gec1 mRNAs, different intensities of labeling were observed depending on the areas: the strongest labeling was observed in the isocortex, hippocampus, basal telencephalon, some thalamic and most of hypothalamic nuclei, cerebellum, and numerous brainstem nuclei. Furthermore, the gec1 mRNAs were intensely expressed in neurons involved in somatomotor and neuroendocrine functions and weakly expressed in sensory and reticular structures. These results corroborate the putative role of the GEC1 protein in the trafficking of receptor GABAA.

Original languageEnglish (US)
Pages (from-to)103-115
Number of pages13
JournalBrain Research
Volume1210
DOIs
StatePublished - May 19 2008

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Central Nervous System
GABA-A Receptors
Messenger RNA
RNA Probes
Neurons
Brain
Proteins
Digoxigenin
Antisense RNA
Telencephalon
Neocortex
3' Untranslated Regions
Protein Transport
Tubulin
Northern Blotting
Cerebellum
Brain Stem
In Situ Hybridization
Real-Time Polymerase Chain Reaction
Hippocampus

Keywords

  • GABA receptor
  • gabarap
  • gec1
  • In situ hybridization
  • Rat brain

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

Cite this

Tolle, F., Risold, P. Y., Mansuy-Schlick, V., Rossi, E., Boyer-Guittaut, M., Fraichard, A., & Jouvenot, M. (2008). Specific regional distribution of gec1 mRNAs in adult rat central nervous system. Brain Research, 1210, 103-115. https://doi.org/10.1016/j.brainres.2008.02.077

Specific regional distribution of gec1 mRNAs in adult rat central nervous system. / Tolle, Fabrice; Risold, Pierre Yves; Mansuy-Schlick, Virginie; Rossi, Emilie; Boyer-Guittaut, Michaël; Fraichard, Annick; Jouvenot, Michèle.

In: Brain Research, Vol. 1210, 19.05.2008, p. 103-115.

Research output: Contribution to journalArticle

Tolle, F, Risold, PY, Mansuy-Schlick, V, Rossi, E, Boyer-Guittaut, M, Fraichard, A & Jouvenot, M 2008, 'Specific regional distribution of gec1 mRNAs in adult rat central nervous system', Brain Research, vol. 1210, pp. 103-115. https://doi.org/10.1016/j.brainres.2008.02.077
Tolle, Fabrice ; Risold, Pierre Yves ; Mansuy-Schlick, Virginie ; Rossi, Emilie ; Boyer-Guittaut, Michaël ; Fraichard, Annick ; Jouvenot, Michèle. / Specific regional distribution of gec1 mRNAs in adult rat central nervous system. In: Brain Research. 2008 ; Vol. 1210. pp. 103-115.
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