The specificity of cell‐mediated lympholysis in the mouse was investigated by studying the cross‐reactivity of in vitro‐generated effector cells against targets carrying H‐2 haplotypes different from that of the stimulator. Congenic resistant partners of the C57BL/10 strain carrying ten different, independently derived haplotypes were tested in more than 600 combinations. Strong cytotoxicity was observed when stimulator and target cells shared a private determinant, but considerable cross‐killing was also seen in combinations where stimulator and target cells shared only public determinants. Cross‐killing is specific and can be blocked only by unlabeled cells of the stimulator or target strain. Although cross‐killing does not correlate with the number of stimulating public determinants carried by the target individual determinants 5, 15. 25, and 27 + 28 + 29 are associated with increased cross‐killing. Cross‐killing can occur both at the K and the D end of H‐2 and is not explained by antigens determined by the I region. We conclude that the serologically defined H‐2K and H‐2D molecules are the primary targets for cytotoxic T lymphocytes but that they may be perceived differently by T and B lymphocytes.
|Original language||English (US)|
|Number of pages||13|
|Journal||Scandinavian Journal of Immunology|
|State||Published - Jul 1975|
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