Specificity of 5lCR-release cytotoxicity of lymphocytes immune to murine sarcoma virus

Ronald B. Herberman, Tadao Aoki, Myrthel Nunn, David H. Lavrin, N. Soares, Adi Gazdar, Howard Holden, K. S S Chang

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

Cellular immune reactivity of C57BL/6 mice after inoculation with murine sarcoma was studied by the15Cr-release cytotoxicity assay. The specificity of the reactions against a tumor target cell induced by Rauscher leukemia virus was examined with an inhibition test. Unlabeled target cells sharing antigens with the labeled target cells could competitively inhibit release of the radioisotope. The results obtained with this inhibition assay correlated well with those obtained with direct cytotoxicity tests against various target cells. C57BL/6 leukemia cells, induced by Friend, Moloney, or Rauscher viruses, could inhibit, and a Gross virus-induced leukemia was negative. However, further results revealed a pattern of specificity different from that seen with serologic reactions. Transplantable BALB/c leukemias, induced by Moloney or Rauscher viruses, gave consistently negative results. Most vlrus-Induced tumors in other species were also negative. Several mouse cell lines spontaneously infected with endogenous C-type viruses were positive, whereas uninfected parental cells were negative. In addition, treatment of a negative cell line with bromodeoxyuridine, an agent causing expression of endogenous viral activity, induced the transient appearance of antigen. This study indicated that the antigens detected by the51Cr-release cytotoxicity assay ot cell-mediated immunity may be related to expression of an endogenous C-type virus, rather than to the murine sarcoma virus. This antigen has been tentatively designated MEV-SA-1. The study distinguished between antigenic specificities detected by humoral antibody and those detected by an assay of cell-mediated immunity.

Original languageEnglish (US)
Pages (from-to)1103-1111
Number of pages9
JournalJournal of the National Cancer Institute
Volume53
Issue number4
DOIs
StatePublished - 1974

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Murine Sarcoma Viruses
Rauscher Virus
Lymphocytes
Antigens
Cellular Immunity
Leukemia
AKR murine leukemia virus
Friend murine leukemia virus
Viruses
Moloney murine leukemia virus
Cell Line
Bromodeoxyuridine
Inbred C57BL Mouse
Radioisotopes
Sarcoma
Epitopes
Neoplasms
Antibodies

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Herberman, R. B., Aoki, T., Nunn, M., Lavrin, D. H., Soares, N., Gazdar, A., ... Chang, K. S. S. (1974). Specificity of 5lCR-release cytotoxicity of lymphocytes immune to murine sarcoma virus. Journal of the National Cancer Institute, 53(4), 1103-1111. https://doi.org/10.1093/jnci/53.4.1103

Specificity of 5lCR-release cytotoxicity of lymphocytes immune to murine sarcoma virus. / Herberman, Ronald B.; Aoki, Tadao; Nunn, Myrthel; Lavrin, David H.; Soares, N.; Gazdar, Adi; Holden, Howard; Chang, K. S S.

In: Journal of the National Cancer Institute, Vol. 53, No. 4, 1974, p. 1103-1111.

Research output: Contribution to journalArticle

Herberman, RB, Aoki, T, Nunn, M, Lavrin, DH, Soares, N, Gazdar, A, Holden, H & Chang, KSS 1974, 'Specificity of 5lCR-release cytotoxicity of lymphocytes immune to murine sarcoma virus', Journal of the National Cancer Institute, vol. 53, no. 4, pp. 1103-1111. https://doi.org/10.1093/jnci/53.4.1103
Herberman, Ronald B. ; Aoki, Tadao ; Nunn, Myrthel ; Lavrin, David H. ; Soares, N. ; Gazdar, Adi ; Holden, Howard ; Chang, K. S S. / Specificity of 5lCR-release cytotoxicity of lymphocytes immune to murine sarcoma virus. In: Journal of the National Cancer Institute. 1974 ; Vol. 53, No. 4. pp. 1103-1111.
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