Spectrophotometric simultaneous determination of manganese(ii) and iron(ii) in pharmaceutical by orthogonal signal correction-partial least squares

Mohammad Goodarzi, Tahmine Goodarzi, Nahid Ghasemi

Research output: Contribution to journalArticle

19 Scopus citations


The simultaneous determination of manganese(II) and iron(II) mixtures by using spectrophotometric methods is a difficult problem in analytical chemistry, due to spectral interferences. By multivariate calibration methods, such as partial least squares (PLS), it is possible to obtain a model adjusted to the concentration values of the mixtures used in the calibration range. Orthogonal signal correction (OSC) is a preprocessing technique used to remove the information unrelated to the target variables based on constrained principal component analysis. OSC is a suitable preprocessing method for partial least squares calibration of mixtures without loss of prediction capacity using spectrophotometric method. In this study, the calibration model is based on absorption spectra in the 450-600 nm range for 21 different mixtures of manganese(II) and iron(II). Calibration matrices were containing 0.05-1.2 and 0.1-2.3 μg mL-1 Mn(II) and Fe(II), respectively. The RMSEP for manganese(II) and iron(II) with OSC and without OSC were 0.0316, 0.0291, and 0.0907, 0.115, respectively. This procedure allows the simultaneous determination of manganese(II) and iron(II) in synthetic and real matrix samples with good reliability of the determination.

Original languageEnglish (US)
Pages (from-to)303-312
Number of pages10
JournalAnnali di Chimica
Issue number5-6
Publication statusPublished - May 1 2007


ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Science(all)

Cite this