Spherical reconstruction

A method for structure determination of membrane proteins from cryo-EM images

Qiu Xing Jiang, David W. Chester, Fred J. Sigworth

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We propose a new method for single-particle reconstruction, which should be generally applicable to structure determination for membrane proteins. After reconstitution into a small spherical vesicle, a membrane protein takes a particular orientation relative to the membrane normal, and its position in the projected image of the vesicle directly defines two of its three Euler angles of orientation. The spherical constraint imposed by the vesicle effectively reduces the dimensionality of the alignment search from 5 to 3 and simplifies the detection of the particle. Projection images of particles in vesicles collectively take all possible orientations and therefore cover the whole Fourier space. Analysis of images of vesicles in ice showed that the vesicle density is well described by a simple model for membrane electron scattering density. In fitting this model we found that osmotically swollen vesicles remain nearly spherical through the freezing process. These results satisfy the basic experimental requirements for spherical reconstruction. A computer simulation of particles in vesicles showed that this method provides good estimates of the two Euler angles and thus may improve single-particle reconstruction and extend it to smaller membrane proteins.

Original languageEnglish (US)
Pages (from-to)119-131
Number of pages13
JournalJournal of Structural Biology
Volume133
Issue number2-3
DOIs
StatePublished - 2001

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Membrane Proteins
Membranes
Ice
Computer Simulation
Freezing
Electrons

Keywords

  • Cryo-electron microscopy
  • Euler angles
  • Membrane proteins
  • Reconstitution
  • Single-particle reconstruction
  • Spherical reconstruction

ASJC Scopus subject areas

  • Structural Biology

Cite this

Spherical reconstruction : A method for structure determination of membrane proteins from cryo-EM images. / Jiang, Qiu Xing; Chester, David W.; Sigworth, Fred J.

In: Journal of Structural Biology, Vol. 133, No. 2-3, 2001, p. 119-131.

Research output: Contribution to journalArticle

Jiang, Qiu Xing ; Chester, David W. ; Sigworth, Fred J. / Spherical reconstruction : A method for structure determination of membrane proteins from cryo-EM images. In: Journal of Structural Biology. 2001 ; Vol. 133, No. 2-3. pp. 119-131.
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