Sphingomyelin exhibits greatly enhanced protection compared with egg yolk phosphatidylcholine against detergent bile salts

Antonio Moschetta, Gerard P. VanBerge-Henegouwen, Piero Portincasa, Giuseppe Palasciano, Albert K. Groen, Karel J. Van Erpecum

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Inclusion of phosphatidylcholine within bile salt micelles protects against bile salt-induced cytotoxicity. In addition to phosphatidylcholine, bile may contain significant amounts of sphingomyelin, particularly under cholestatic conditions. We compared protective effects of egg yolk phosphatidylcholine (similar to phosphatidylcholine in bile), egg yolk sphingomyelin (mainly 16:0 acyl chains) and dipalmitoyl phosphatidylcholine against taurocholate in complementary in vitro studies. Upon addition of taurocholate-containing micelles to sonicated egg yolk phosphatidylcholine vesicles, subsequent micellization of the vesicular bilayer proved to be retarded when phospholipids had also been included in these micelles in the rank order: egg yolk phosphatidylcholine < dipalmitoyl phosphatidylcholine < sphingomyelin. Hemolysis of erythrocytes and LDH release by CaCo-2 cells after addition of taurocholate micelles were strongly reduced by including small amounts of sphingomyelin or dipalmitoyl phosphatidylcholine in these micelles (PL/(PL + BS) ≥ 0.1), whereas egg yolk phosphatidylcholine provided less protection. Amounts of non-phospholipid-associated bile salts (thought to be responsible for cytotoxicity) in egg yolk phosphatidylcholine- containing micelles were significantly higher than in corresponding sphingomyelinor dipalmitoyl phosphatidylcholine-containing micelles (tested at PL/(PL + BS) ratios 0.1, 0.15, and 0.2). LDH release upon incubation of CaCo-2 cells with taurocholate simple micelles at these so-called 'intermixed micellar-vesicular' concentrations was identical to LDH release upon incubation with corresponding taurocholate-phospholipid mixed micelles. In conclusion, we found greatly enhanced protective effects of sphingomyelin and dipalmitoyl phosphatidylcholine compared to egg yolk phosphatidylcholine against bile salt-induced cytotoxicity, related to different amounts of non- phospholipid-associated bile salts. These findings may be relevant for protection against bile salt-induced cytotoxicity in vivo.

Original languageEnglish (US)
Pages (from-to)916-924
Number of pages9
JournalJournal of Lipid Research
Volume41
Issue number6
StatePublished - Jun 2000

Fingerprint

Egg Yolk
Sphingomyelins
Micelles
Bile Acids and Salts
Phosphatidylcholines
Detergents
1,2-Dipalmitoylphosphatidylcholine
Taurocholic Acid
Cytotoxicity
Phospholipids
Caco-2 Cells
Bile
Micellization
Hemolysis
Erythrocytes

Keywords

  • Bile salts
  • CaCo-2 cells
  • Erythrocytes
  • Intermixed micellar-vesicular bile salt concentration
  • Micelles
  • Phosphatidylcholine
  • Phospholipids
  • Sphingomyelin
  • Vesicl es

ASJC Scopus subject areas

  • Endocrinology

Cite this

Moschetta, A., VanBerge-Henegouwen, G. P., Portincasa, P., Palasciano, G., Groen, A. K., & Van Erpecum, K. J. (2000). Sphingomyelin exhibits greatly enhanced protection compared with egg yolk phosphatidylcholine against detergent bile salts. Journal of Lipid Research, 41(6), 916-924.

Sphingomyelin exhibits greatly enhanced protection compared with egg yolk phosphatidylcholine against detergent bile salts. / Moschetta, Antonio; VanBerge-Henegouwen, Gerard P.; Portincasa, Piero; Palasciano, Giuseppe; Groen, Albert K.; Van Erpecum, Karel J.

In: Journal of Lipid Research, Vol. 41, No. 6, 06.2000, p. 916-924.

Research output: Contribution to journalArticle

Moschetta, A, VanBerge-Henegouwen, GP, Portincasa, P, Palasciano, G, Groen, AK & Van Erpecum, KJ 2000, 'Sphingomyelin exhibits greatly enhanced protection compared with egg yolk phosphatidylcholine against detergent bile salts', Journal of Lipid Research, vol. 41, no. 6, pp. 916-924.
Moschetta A, VanBerge-Henegouwen GP, Portincasa P, Palasciano G, Groen AK, Van Erpecum KJ. Sphingomyelin exhibits greatly enhanced protection compared with egg yolk phosphatidylcholine against detergent bile salts. Journal of Lipid Research. 2000 Jun;41(6):916-924.
Moschetta, Antonio ; VanBerge-Henegouwen, Gerard P. ; Portincasa, Piero ; Palasciano, Giuseppe ; Groen, Albert K. ; Van Erpecum, Karel J. / Sphingomyelin exhibits greatly enhanced protection compared with egg yolk phosphatidylcholine against detergent bile salts. In: Journal of Lipid Research. 2000 ; Vol. 41, No. 6. pp. 916-924.
@article{7a3bac0337b944f0a9d285e8ddb25f7a,
title = "Sphingomyelin exhibits greatly enhanced protection compared with egg yolk phosphatidylcholine against detergent bile salts",
abstract = "Inclusion of phosphatidylcholine within bile salt micelles protects against bile salt-induced cytotoxicity. In addition to phosphatidylcholine, bile may contain significant amounts of sphingomyelin, particularly under cholestatic conditions. We compared protective effects of egg yolk phosphatidylcholine (similar to phosphatidylcholine in bile), egg yolk sphingomyelin (mainly 16:0 acyl chains) and dipalmitoyl phosphatidylcholine against taurocholate in complementary in vitro studies. Upon addition of taurocholate-containing micelles to sonicated egg yolk phosphatidylcholine vesicles, subsequent micellization of the vesicular bilayer proved to be retarded when phospholipids had also been included in these micelles in the rank order: egg yolk phosphatidylcholine < dipalmitoyl phosphatidylcholine < sphingomyelin. Hemolysis of erythrocytes and LDH release by CaCo-2 cells after addition of taurocholate micelles were strongly reduced by including small amounts of sphingomyelin or dipalmitoyl phosphatidylcholine in these micelles (PL/(PL + BS) ≥ 0.1), whereas egg yolk phosphatidylcholine provided less protection. Amounts of non-phospholipid-associated bile salts (thought to be responsible for cytotoxicity) in egg yolk phosphatidylcholine- containing micelles were significantly higher than in corresponding sphingomyelinor dipalmitoyl phosphatidylcholine-containing micelles (tested at PL/(PL + BS) ratios 0.1, 0.15, and 0.2). LDH release upon incubation of CaCo-2 cells with taurocholate simple micelles at these so-called 'intermixed micellar-vesicular' concentrations was identical to LDH release upon incubation with corresponding taurocholate-phospholipid mixed micelles. In conclusion, we found greatly enhanced protective effects of sphingomyelin and dipalmitoyl phosphatidylcholine compared to egg yolk phosphatidylcholine against bile salt-induced cytotoxicity, related to different amounts of non- phospholipid-associated bile salts. These findings may be relevant for protection against bile salt-induced cytotoxicity in vivo.",
keywords = "Bile salts, CaCo-2 cells, Erythrocytes, Intermixed micellar-vesicular bile salt concentration, Micelles, Phosphatidylcholine, Phospholipids, Sphingomyelin, Vesicl es",
author = "Antonio Moschetta and VanBerge-Henegouwen, {Gerard P.} and Piero Portincasa and Giuseppe Palasciano and Groen, {Albert K.} and {Van Erpecum}, {Karel J.}",
year = "2000",
month = "6",
language = "English (US)",
volume = "41",
pages = "916--924",
journal = "Journal of Lipid Research",
issn = "0022-2275",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "6",

}

TY - JOUR

T1 - Sphingomyelin exhibits greatly enhanced protection compared with egg yolk phosphatidylcholine against detergent bile salts

AU - Moschetta, Antonio

AU - VanBerge-Henegouwen, Gerard P.

AU - Portincasa, Piero

AU - Palasciano, Giuseppe

AU - Groen, Albert K.

AU - Van Erpecum, Karel J.

PY - 2000/6

Y1 - 2000/6

N2 - Inclusion of phosphatidylcholine within bile salt micelles protects against bile salt-induced cytotoxicity. In addition to phosphatidylcholine, bile may contain significant amounts of sphingomyelin, particularly under cholestatic conditions. We compared protective effects of egg yolk phosphatidylcholine (similar to phosphatidylcholine in bile), egg yolk sphingomyelin (mainly 16:0 acyl chains) and dipalmitoyl phosphatidylcholine against taurocholate in complementary in vitro studies. Upon addition of taurocholate-containing micelles to sonicated egg yolk phosphatidylcholine vesicles, subsequent micellization of the vesicular bilayer proved to be retarded when phospholipids had also been included in these micelles in the rank order: egg yolk phosphatidylcholine < dipalmitoyl phosphatidylcholine < sphingomyelin. Hemolysis of erythrocytes and LDH release by CaCo-2 cells after addition of taurocholate micelles were strongly reduced by including small amounts of sphingomyelin or dipalmitoyl phosphatidylcholine in these micelles (PL/(PL + BS) ≥ 0.1), whereas egg yolk phosphatidylcholine provided less protection. Amounts of non-phospholipid-associated bile salts (thought to be responsible for cytotoxicity) in egg yolk phosphatidylcholine- containing micelles were significantly higher than in corresponding sphingomyelinor dipalmitoyl phosphatidylcholine-containing micelles (tested at PL/(PL + BS) ratios 0.1, 0.15, and 0.2). LDH release upon incubation of CaCo-2 cells with taurocholate simple micelles at these so-called 'intermixed micellar-vesicular' concentrations was identical to LDH release upon incubation with corresponding taurocholate-phospholipid mixed micelles. In conclusion, we found greatly enhanced protective effects of sphingomyelin and dipalmitoyl phosphatidylcholine compared to egg yolk phosphatidylcholine against bile salt-induced cytotoxicity, related to different amounts of non- phospholipid-associated bile salts. These findings may be relevant for protection against bile salt-induced cytotoxicity in vivo.

AB - Inclusion of phosphatidylcholine within bile salt micelles protects against bile salt-induced cytotoxicity. In addition to phosphatidylcholine, bile may contain significant amounts of sphingomyelin, particularly under cholestatic conditions. We compared protective effects of egg yolk phosphatidylcholine (similar to phosphatidylcholine in bile), egg yolk sphingomyelin (mainly 16:0 acyl chains) and dipalmitoyl phosphatidylcholine against taurocholate in complementary in vitro studies. Upon addition of taurocholate-containing micelles to sonicated egg yolk phosphatidylcholine vesicles, subsequent micellization of the vesicular bilayer proved to be retarded when phospholipids had also been included in these micelles in the rank order: egg yolk phosphatidylcholine < dipalmitoyl phosphatidylcholine < sphingomyelin. Hemolysis of erythrocytes and LDH release by CaCo-2 cells after addition of taurocholate micelles were strongly reduced by including small amounts of sphingomyelin or dipalmitoyl phosphatidylcholine in these micelles (PL/(PL + BS) ≥ 0.1), whereas egg yolk phosphatidylcholine provided less protection. Amounts of non-phospholipid-associated bile salts (thought to be responsible for cytotoxicity) in egg yolk phosphatidylcholine- containing micelles were significantly higher than in corresponding sphingomyelinor dipalmitoyl phosphatidylcholine-containing micelles (tested at PL/(PL + BS) ratios 0.1, 0.15, and 0.2). LDH release upon incubation of CaCo-2 cells with taurocholate simple micelles at these so-called 'intermixed micellar-vesicular' concentrations was identical to LDH release upon incubation with corresponding taurocholate-phospholipid mixed micelles. In conclusion, we found greatly enhanced protective effects of sphingomyelin and dipalmitoyl phosphatidylcholine compared to egg yolk phosphatidylcholine against bile salt-induced cytotoxicity, related to different amounts of non- phospholipid-associated bile salts. These findings may be relevant for protection against bile salt-induced cytotoxicity in vivo.

KW - Bile salts

KW - CaCo-2 cells

KW - Erythrocytes

KW - Intermixed micellar-vesicular bile salt concentration

KW - Micelles

KW - Phosphatidylcholine

KW - Phospholipids

KW - Sphingomyelin

KW - Vesicl es

UR - http://www.scopus.com/inward/record.url?scp=0033935502&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033935502&partnerID=8YFLogxK

M3 - Article

VL - 41

SP - 916

EP - 924

JO - Journal of Lipid Research

JF - Journal of Lipid Research

SN - 0022-2275

IS - 6

ER -