Abstract
Inclusion of phosphatidylcholine within bile salt micelles protects against bile salt-induced cytotoxicity. In addition to phosphatidylcholine, bile may contain significant amounts of sphingomyelin, particularly under cholestatic conditions. We compared protective effects of egg yolk phosphatidylcholine (similar to phosphatidylcholine in bile), egg yolk sphingomyelin (mainly 16:0 acyl chains) and dipalmitoyl phosphatidylcholine against taurocholate in complementary in vitro studies. Upon addition of taurocholate-containing micelles to sonicated egg yolk phosphatidylcholine vesicles, subsequent micellization of the vesicular bilayer proved to be retarded when phospholipids had also been included in these micelles in the rank order: egg yolk phosphatidylcholine < dipalmitoyl phosphatidylcholine < sphingomyelin. Hemolysis of erythrocytes and LDH release by CaCo-2 cells after addition of taurocholate micelles were strongly reduced by including small amounts of sphingomyelin or dipalmitoyl phosphatidylcholine in these micelles (PL/(PL + BS) ≥ 0.1), whereas egg yolk phosphatidylcholine provided less protection. Amounts of non-phospholipid-associated bile salts (thought to be responsible for cytotoxicity) in egg yolk phosphatidylcholine- containing micelles were significantly higher than in corresponding sphingomyelinor dipalmitoyl phosphatidylcholine-containing micelles (tested at PL/(PL + BS) ratios 0.1, 0.15, and 0.2). LDH release upon incubation of CaCo-2 cells with taurocholate simple micelles at these so-called 'intermixed micellar-vesicular' concentrations was identical to LDH release upon incubation with corresponding taurocholate-phospholipid mixed micelles. In conclusion, we found greatly enhanced protective effects of sphingomyelin and dipalmitoyl phosphatidylcholine compared to egg yolk phosphatidylcholine against bile salt-induced cytotoxicity, related to different amounts of non- phospholipid-associated bile salts. These findings may be relevant for protection against bile salt-induced cytotoxicity in vivo.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 916-924 |
| Number of pages | 9 |
| Journal | Journal of Lipid Research |
| Volume | 41 |
| Issue number | 6 |
| State | Published - Jun 2000 |
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Keywords
- Bile salts
- CaCo-2 cells
- Erythrocytes
- Intermixed micellar-vesicular bile salt concentration
- Micelles
- Phosphatidylcholine
- Phospholipids
- Sphingomyelin
- Vesicl es
ASJC Scopus subject areas
- Endocrinology
Cite this
Sphingomyelin exhibits greatly enhanced protection compared with egg yolk phosphatidylcholine against detergent bile salts. / Moschetta, Antonio; VanBerge-Henegouwen, Gerard P.; Portincasa, Piero; Palasciano, Giuseppe; Groen, Albert K.; Van Erpecum, Karel J.
In: Journal of Lipid Research, Vol. 41, No. 6, 06.2000, p. 916-924.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sphingomyelin exhibits greatly enhanced protection compared with egg yolk phosphatidylcholine against detergent bile salts
AU - Moschetta, Antonio
AU - VanBerge-Henegouwen, Gerard P.
AU - Portincasa, Piero
AU - Palasciano, Giuseppe
AU - Groen, Albert K.
AU - Van Erpecum, Karel J.
PY - 2000/6
Y1 - 2000/6
N2 - Inclusion of phosphatidylcholine within bile salt micelles protects against bile salt-induced cytotoxicity. In addition to phosphatidylcholine, bile may contain significant amounts of sphingomyelin, particularly under cholestatic conditions. We compared protective effects of egg yolk phosphatidylcholine (similar to phosphatidylcholine in bile), egg yolk sphingomyelin (mainly 16:0 acyl chains) and dipalmitoyl phosphatidylcholine against taurocholate in complementary in vitro studies. Upon addition of taurocholate-containing micelles to sonicated egg yolk phosphatidylcholine vesicles, subsequent micellization of the vesicular bilayer proved to be retarded when phospholipids had also been included in these micelles in the rank order: egg yolk phosphatidylcholine < dipalmitoyl phosphatidylcholine < sphingomyelin. Hemolysis of erythrocytes and LDH release by CaCo-2 cells after addition of taurocholate micelles were strongly reduced by including small amounts of sphingomyelin or dipalmitoyl phosphatidylcholine in these micelles (PL/(PL + BS) ≥ 0.1), whereas egg yolk phosphatidylcholine provided less protection. Amounts of non-phospholipid-associated bile salts (thought to be responsible for cytotoxicity) in egg yolk phosphatidylcholine- containing micelles were significantly higher than in corresponding sphingomyelinor dipalmitoyl phosphatidylcholine-containing micelles (tested at PL/(PL + BS) ratios 0.1, 0.15, and 0.2). LDH release upon incubation of CaCo-2 cells with taurocholate simple micelles at these so-called 'intermixed micellar-vesicular' concentrations was identical to LDH release upon incubation with corresponding taurocholate-phospholipid mixed micelles. In conclusion, we found greatly enhanced protective effects of sphingomyelin and dipalmitoyl phosphatidylcholine compared to egg yolk phosphatidylcholine against bile salt-induced cytotoxicity, related to different amounts of non- phospholipid-associated bile salts. These findings may be relevant for protection against bile salt-induced cytotoxicity in vivo.
AB - Inclusion of phosphatidylcholine within bile salt micelles protects against bile salt-induced cytotoxicity. In addition to phosphatidylcholine, bile may contain significant amounts of sphingomyelin, particularly under cholestatic conditions. We compared protective effects of egg yolk phosphatidylcholine (similar to phosphatidylcholine in bile), egg yolk sphingomyelin (mainly 16:0 acyl chains) and dipalmitoyl phosphatidylcholine against taurocholate in complementary in vitro studies. Upon addition of taurocholate-containing micelles to sonicated egg yolk phosphatidylcholine vesicles, subsequent micellization of the vesicular bilayer proved to be retarded when phospholipids had also been included in these micelles in the rank order: egg yolk phosphatidylcholine < dipalmitoyl phosphatidylcholine < sphingomyelin. Hemolysis of erythrocytes and LDH release by CaCo-2 cells after addition of taurocholate micelles were strongly reduced by including small amounts of sphingomyelin or dipalmitoyl phosphatidylcholine in these micelles (PL/(PL + BS) ≥ 0.1), whereas egg yolk phosphatidylcholine provided less protection. Amounts of non-phospholipid-associated bile salts (thought to be responsible for cytotoxicity) in egg yolk phosphatidylcholine- containing micelles were significantly higher than in corresponding sphingomyelinor dipalmitoyl phosphatidylcholine-containing micelles (tested at PL/(PL + BS) ratios 0.1, 0.15, and 0.2). LDH release upon incubation of CaCo-2 cells with taurocholate simple micelles at these so-called 'intermixed micellar-vesicular' concentrations was identical to LDH release upon incubation with corresponding taurocholate-phospholipid mixed micelles. In conclusion, we found greatly enhanced protective effects of sphingomyelin and dipalmitoyl phosphatidylcholine compared to egg yolk phosphatidylcholine against bile salt-induced cytotoxicity, related to different amounts of non- phospholipid-associated bile salts. These findings may be relevant for protection against bile salt-induced cytotoxicity in vivo.
KW - Bile salts
KW - CaCo-2 cells
KW - Erythrocytes
KW - Intermixed micellar-vesicular bile salt concentration
KW - Micelles
KW - Phosphatidylcholine
KW - Phospholipids
KW - Sphingomyelin
KW - Vesicl es
UR - http://www.scopus.com/inward/record.url?scp=0033935502&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033935502&partnerID=8YFLogxK
M3 - Article
VL - 41
SP - 916
EP - 924
JO - Journal of Lipid Research
JF - Journal of Lipid Research
SN - 0022-2275
IS - 6
ER -