Sphingomyelin protects against apoptosis and hyperproliferation induced by deoxycholate: Potential implications for colon cancer

A. Moschetta, Piero Portincasa, K. J. Van Erpecum, L. Debellis, G. P. Vanberge-Henegouwen, G. Palasciano

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

High fecal deoxycholate levels may promote colonic cancer. Phospholipids protect against bile salt-induced cytotoxicity. We therefore aimed to examine whether the dietary phospholipid sphingomyelin could decrease hyperproliferation induced by deoxycholate. In CaCo2 cells, hyperproliferation (by bromodeoxyuridine assay), phosphorylation state of cellular proteins, and apoptosis with concomitant caspase-3 activity were evaluated after incubation with 50-500 μM deoxycholate, with or without sphingomyelin. At 2 and 4 hr of incubation, deoxycholate induced dose-dependent apoptosis, with concomitant caspase-3 activation. At 16 hr, apoptosis had decreased markedly, but there was dose-dependent hyperproliferation (with changed phosphorylation status of cellular proteins) at this time point. Sphingomyelin dose-dependently reduced deoxycholate-induced apoptosis and hyperproliferation. In conclusion, sphingomyelin reduces deoxycholate-induced hyperproliferation and apoptosis. These findings may have implications for colonic cancer prevention by dietary modification.

Original languageEnglish (US)
Pages (from-to)1094-1101
Number of pages8
JournalDigestive Diseases and Sciences
Volume48
Issue number6
DOIs
StatePublished - Jun 1 2003

Keywords

  • Bile salts
  • Colon cancer
  • Phospholipids
  • Prevention

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

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    Moschetta, A., Portincasa, P., Van Erpecum, K. J., Debellis, L., Vanberge-Henegouwen, G. P., & Palasciano, G. (2003). Sphingomyelin protects against apoptosis and hyperproliferation induced by deoxycholate: Potential implications for colon cancer. Digestive Diseases and Sciences, 48(6), 1094-1101. https://doi.org/10.1023/A:1023712712025