TY - JOUR
T1 - Splice variants of the α subunit of the G protein Gs activate both adenylyl cyclase and calcium channels
AU - Mattera, Rafael
AU - Graziano, Michael P.
AU - Yatani, Atsuko
AU - Zhou, Zhimin
AU - Graf, Rolf
AU - Codina, Juan
AU - Birnbaumer, Lutz
AU - Gilman, Alfred G.
AU - Brown, Arthur M.
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1989
Y1 - 1989
N2 - Signal transducing guanine nucleotide binding (G) proteins are heterotrimers with different α subunits that confer specificity for interactions with receptors and effectors. Eight to ten such G proteins couple a large number of receptors for hormones and neurotransmitters to at least eight different effectors. Although one G protein can interact with several receptors, a given G protein was thought to interact with but one effector. The recent finding that voltage-gated calcium channels are stimulated by purified G s, which stimulates adenylyl cyclase, challenged this concept. However, purified Gs may have four distinct α-subunit polypeptides, produced by alternative splicing of messenger RNA. By using recombinant DNA techniques, three of the splice variants were synthesized in Escherichia coli and each variant was shown to stimulate both adenylyl cyclase and calcium channels. Thus, a single G protein α subunit may regulate more than one effector function.
AB - Signal transducing guanine nucleotide binding (G) proteins are heterotrimers with different α subunits that confer specificity for interactions with receptors and effectors. Eight to ten such G proteins couple a large number of receptors for hormones and neurotransmitters to at least eight different effectors. Although one G protein can interact with several receptors, a given G protein was thought to interact with but one effector. The recent finding that voltage-gated calcium channels are stimulated by purified G s, which stimulates adenylyl cyclase, challenged this concept. However, purified Gs may have four distinct α-subunit polypeptides, produced by alternative splicing of messenger RNA. By using recombinant DNA techniques, three of the splice variants were synthesized in Escherichia coli and each variant was shown to stimulate both adenylyl cyclase and calcium channels. Thus, a single G protein α subunit may regulate more than one effector function.
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U2 - 10.1126/science.2536957
DO - 10.1126/science.2536957
M3 - Article
C2 - 2536957
AN - SCOPUS:0024539357
SN - 0036-8075
VL - 243
SP - 804
EP - 807
JO - Science
JF - Science
IS - 4892
ER -