Spontaneous expression of murine endogenous viruses in hamster x mouse hybrid cells

S. S. Park, A. F. Gazdar, P. A. Lalley, J. D. Minna, U. Francke

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The spontaneous expression patterns of murine leukemia virus (MuLV) from mouse spleen cultures and hamster x mouse spleen hybrid cells were compared. The hybrid cells preferentially segregated mouse while retaining a complete set of hamster chromosomes. Virus expression patterns showed considerable mouse strain differences in both parental and hybrid cells. None of 13 BALB/c spleen cultures expressed virus while 6 of 20 hybrid clones expressed N-tropic or N-tropic and X-tropic viruses. AII AKR spleen cultures and 5 of 7 hybrids expressed N-tropic but not X-tropic virus. X-tropic virus expression was detected in all 6 NZB spleen cultures and in 5 of 8 hybrids. Virus expression did not occur in NIH Swiss or NIH Swiss/Nude (nu/nu) spleen or hybrid cultures. A major structural locus (Akv-1) for N-tropic virus expression has been assigned to chromosome 7 in AKR mice by recombinational genetics and is closely linked to the gene for glucose phosphate isomerase (Gpi-1). Analysis of AKR hybrids confirmed the requirement of chromosome 7 for initial expression of mouse N-tropic virus and, in addition, excluded the role of all other mouse chromosomes in this phenomenon. Eighteen of 20 BALB/c clones retained chromosome 7, including 6 virus-positive clones, suggesting that chromosome 7 may be necessary but is not sufficient for N-tropic virus expression in this strain. Hybrid clones with examples of asynteny were found for all other chromosomes. Long-term passage and subcloning studies of AKR and BALB/c virus-positive hybrids indicated that virus-positive clones segregated independently of chromosome 7 markers. Spontaneous expression of X-tropic virus in NZB hybrid clones was syntenic with the gene for adenylate kinase (Ak-1) which is assigned to chromosome 2. A relatively small number of NZB hybrid clones were studied and, a firm gene assignment cannot be made, because only one discordant clone each was found for chromosomes 1, 3, 10, 19. Our studies indicate that spontaneous expression of MuLV strains does not require the following MuLV-related genes: Fv-1 (chromosome 4), Fv-2 (chromosome 9), Rec-1 (replication of ecotropic virus, chromosome 5), Gv-1 and H-2 (chromosome 17).

Original languageEnglish (US)
Pages (from-to)52-61
Number of pages10
JournalInternational Journal of Cancer
Volume23
Issue number1
StatePublished - 1979

Fingerprint

Hybrid Cells
Cricetinae
Viruses
Clone Cells
Chromosomes, Human, Pair 7
Spleen
Murine Leukemia Viruses
Chromosomes
xylose isomerase
Genes
Inbred AKR Mouse
Chromosomes, Human, Pair 9
Chromosomes, Human, Pair 5
Chromosomes, Human, Pair 17
Chromosomes, Human, Pair 4
Chromosomes, Human, Pair 3
Chromosomes, Human, Pair 2
Chromosomes, Human, Pair 1
Virus Replication
Genetic Markers

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Spontaneous expression of murine endogenous viruses in hamster x mouse hybrid cells. / Park, S. S.; Gazdar, A. F.; Lalley, P. A.; Minna, J. D.; Francke, U.

In: International Journal of Cancer, Vol. 23, No. 1, 1979, p. 52-61.

Research output: Contribution to journalArticle

@article{6781c4fed56f4956bbf9aea4bafd7490,
title = "Spontaneous expression of murine endogenous viruses in hamster x mouse hybrid cells",
abstract = "The spontaneous expression patterns of murine leukemia virus (MuLV) from mouse spleen cultures and hamster x mouse spleen hybrid cells were compared. The hybrid cells preferentially segregated mouse while retaining a complete set of hamster chromosomes. Virus expression patterns showed considerable mouse strain differences in both parental and hybrid cells. None of 13 BALB/c spleen cultures expressed virus while 6 of 20 hybrid clones expressed N-tropic or N-tropic and X-tropic viruses. AII AKR spleen cultures and 5 of 7 hybrids expressed N-tropic but not X-tropic virus. X-tropic virus expression was detected in all 6 NZB spleen cultures and in 5 of 8 hybrids. Virus expression did not occur in NIH Swiss or NIH Swiss/Nude (nu/nu) spleen or hybrid cultures. A major structural locus (Akv-1) for N-tropic virus expression has been assigned to chromosome 7 in AKR mice by recombinational genetics and is closely linked to the gene for glucose phosphate isomerase (Gpi-1). Analysis of AKR hybrids confirmed the requirement of chromosome 7 for initial expression of mouse N-tropic virus and, in addition, excluded the role of all other mouse chromosomes in this phenomenon. Eighteen of 20 BALB/c clones retained chromosome 7, including 6 virus-positive clones, suggesting that chromosome 7 may be necessary but is not sufficient for N-tropic virus expression in this strain. Hybrid clones with examples of asynteny were found for all other chromosomes. Long-term passage and subcloning studies of AKR and BALB/c virus-positive hybrids indicated that virus-positive clones segregated independently of chromosome 7 markers. Spontaneous expression of X-tropic virus in NZB hybrid clones was syntenic with the gene for adenylate kinase (Ak-1) which is assigned to chromosome 2. A relatively small number of NZB hybrid clones were studied and, a firm gene assignment cannot be made, because only one discordant clone each was found for chromosomes 1, 3, 10, 19. Our studies indicate that spontaneous expression of MuLV strains does not require the following MuLV-related genes: Fv-1 (chromosome 4), Fv-2 (chromosome 9), Rec-1 (replication of ecotropic virus, chromosome 5), Gv-1 and H-2 (chromosome 17).",
author = "Park, {S. S.} and Gazdar, {A. F.} and Lalley, {P. A.} and Minna, {J. D.} and U. Francke",
year = "1979",
language = "English (US)",
volume = "23",
pages = "52--61",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Spontaneous expression of murine endogenous viruses in hamster x mouse hybrid cells

AU - Park, S. S.

AU - Gazdar, A. F.

AU - Lalley, P. A.

AU - Minna, J. D.

AU - Francke, U.

PY - 1979

Y1 - 1979

N2 - The spontaneous expression patterns of murine leukemia virus (MuLV) from mouse spleen cultures and hamster x mouse spleen hybrid cells were compared. The hybrid cells preferentially segregated mouse while retaining a complete set of hamster chromosomes. Virus expression patterns showed considerable mouse strain differences in both parental and hybrid cells. None of 13 BALB/c spleen cultures expressed virus while 6 of 20 hybrid clones expressed N-tropic or N-tropic and X-tropic viruses. AII AKR spleen cultures and 5 of 7 hybrids expressed N-tropic but not X-tropic virus. X-tropic virus expression was detected in all 6 NZB spleen cultures and in 5 of 8 hybrids. Virus expression did not occur in NIH Swiss or NIH Swiss/Nude (nu/nu) spleen or hybrid cultures. A major structural locus (Akv-1) for N-tropic virus expression has been assigned to chromosome 7 in AKR mice by recombinational genetics and is closely linked to the gene for glucose phosphate isomerase (Gpi-1). Analysis of AKR hybrids confirmed the requirement of chromosome 7 for initial expression of mouse N-tropic virus and, in addition, excluded the role of all other mouse chromosomes in this phenomenon. Eighteen of 20 BALB/c clones retained chromosome 7, including 6 virus-positive clones, suggesting that chromosome 7 may be necessary but is not sufficient for N-tropic virus expression in this strain. Hybrid clones with examples of asynteny were found for all other chromosomes. Long-term passage and subcloning studies of AKR and BALB/c virus-positive hybrids indicated that virus-positive clones segregated independently of chromosome 7 markers. Spontaneous expression of X-tropic virus in NZB hybrid clones was syntenic with the gene for adenylate kinase (Ak-1) which is assigned to chromosome 2. A relatively small number of NZB hybrid clones were studied and, a firm gene assignment cannot be made, because only one discordant clone each was found for chromosomes 1, 3, 10, 19. Our studies indicate that spontaneous expression of MuLV strains does not require the following MuLV-related genes: Fv-1 (chromosome 4), Fv-2 (chromosome 9), Rec-1 (replication of ecotropic virus, chromosome 5), Gv-1 and H-2 (chromosome 17).

AB - The spontaneous expression patterns of murine leukemia virus (MuLV) from mouse spleen cultures and hamster x mouse spleen hybrid cells were compared. The hybrid cells preferentially segregated mouse while retaining a complete set of hamster chromosomes. Virus expression patterns showed considerable mouse strain differences in both parental and hybrid cells. None of 13 BALB/c spleen cultures expressed virus while 6 of 20 hybrid clones expressed N-tropic or N-tropic and X-tropic viruses. AII AKR spleen cultures and 5 of 7 hybrids expressed N-tropic but not X-tropic virus. X-tropic virus expression was detected in all 6 NZB spleen cultures and in 5 of 8 hybrids. Virus expression did not occur in NIH Swiss or NIH Swiss/Nude (nu/nu) spleen or hybrid cultures. A major structural locus (Akv-1) for N-tropic virus expression has been assigned to chromosome 7 in AKR mice by recombinational genetics and is closely linked to the gene for glucose phosphate isomerase (Gpi-1). Analysis of AKR hybrids confirmed the requirement of chromosome 7 for initial expression of mouse N-tropic virus and, in addition, excluded the role of all other mouse chromosomes in this phenomenon. Eighteen of 20 BALB/c clones retained chromosome 7, including 6 virus-positive clones, suggesting that chromosome 7 may be necessary but is not sufficient for N-tropic virus expression in this strain. Hybrid clones with examples of asynteny were found for all other chromosomes. Long-term passage and subcloning studies of AKR and BALB/c virus-positive hybrids indicated that virus-positive clones segregated independently of chromosome 7 markers. Spontaneous expression of X-tropic virus in NZB hybrid clones was syntenic with the gene for adenylate kinase (Ak-1) which is assigned to chromosome 2. A relatively small number of NZB hybrid clones were studied and, a firm gene assignment cannot be made, because only one discordant clone each was found for chromosomes 1, 3, 10, 19. Our studies indicate that spontaneous expression of MuLV strains does not require the following MuLV-related genes: Fv-1 (chromosome 4), Fv-2 (chromosome 9), Rec-1 (replication of ecotropic virus, chromosome 5), Gv-1 and H-2 (chromosome 17).

UR - http://www.scopus.com/inward/record.url?scp=0018350893&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018350893&partnerID=8YFLogxK

M3 - Article

VL - 23

SP - 52

EP - 61

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 1

ER -