SRY-box-containing gene 2 regulation of nuclear receptor tailless (Tlx) transcription in adult neural stem cells

Koji Shimozaki, Chun Li Zhang, Hoonkyo Suh, Ahmet M. Denli, Ronald M. Evans, Fred H. Gage

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Adult neurogenesis is maintained by self-renewable neural stem cells (NSCs). Their activity is regulated by multiple signaling pathways and key transcription factors. However, it has been unclear whether these factors interplay with each other at the molecular level. Here we show that SRY-box-containing gene 2 (Sox2) and nuclear receptor tailless (TLX) form a molecular network in adult NSCs. We observed that both Sox2 and TLX proteins bind to the upstream region of Tlx gene. Sox2 positively regulates Tlx expression, whereas the binding of TLX to its own promoter suppresses its transcriptional activity in luciferase reporter assays. Such TLX-mediated suppression can be antagonized by overexpressing wild-type Sox2 but not a mutant lacking the transcriptional activation domain. Furthermore, through regions involved in DNA-binding activity, Sox2 and TLX physically interact to form a complex on DNAs that contain a consensus binding site for TLX. Finally, depletion of Sox2 revealed the potential negative feedback loop of TLX expression that is antagonized by Sox2 in adult NSCs. These data suggest that Sox2 plays an important role in Tlx transcription in cultured adult NSCs.

Original languageEnglish (US)
Pages (from-to)5969-5978
Number of pages10
JournalJournal of Biological Chemistry
Volume287
Issue number8
DOIs
StatePublished - Feb 17 2012

Fingerprint

Adult Stem Cells
Neural Stem Cells
Transcription
Cytoplasmic and Nuclear Receptors
Stem cells
Genes
DNA
Neurogenesis
Luciferases
Transcriptional Activation
Assays
Transcription Factors
Chemical activation
Binding Sites
Feedback
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

SRY-box-containing gene 2 regulation of nuclear receptor tailless (Tlx) transcription in adult neural stem cells. / Shimozaki, Koji; Zhang, Chun Li; Suh, Hoonkyo; Denli, Ahmet M.; Evans, Ronald M.; Gage, Fred H.

In: Journal of Biological Chemistry, Vol. 287, No. 8, 17.02.2012, p. 5969-5978.

Research output: Contribution to journalArticle

Shimozaki, Koji ; Zhang, Chun Li ; Suh, Hoonkyo ; Denli, Ahmet M. ; Evans, Ronald M. ; Gage, Fred H. / SRY-box-containing gene 2 regulation of nuclear receptor tailless (Tlx) transcription in adult neural stem cells. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 8. pp. 5969-5978.
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