Stable metal oxide nanoparticle formulation for toxicity studies

Deepak Gurbani, Ritesh K. Shukla, Alok K. Pandey, Alok Dhawan

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Metal oxide nanoparticles such as TiO 2 (<100 nm) are used in lotions and sunscreens. Toxicity studies so far have reported the use of TiO 2 NPs with size >100 nm as determined by dynamic light scattering (DLS) technique. We used non-reactive chemical agents such as propylene glycol (PG), glycerol (G), ethylene glycol (EG) to prevent aggregation and maintain NPs in monodispersed state closer to the reported TEM size. The lowest concentration of PG (0.1%) along with complete culture media (RPMI 1640) showed mean hydrodynamic diameter of TiO 2 NPs as 33.71 nm measured by DLS. The present study demonstrates a method to prepare stable TiO 2 NPs in culture media for toxicity assessment. This is a significant contribution as the biological properties of NPs vary considerably with decrease in size.

Original languageEnglish (US)
Pages (from-to)104-105
Number of pages2
JournalJournal of biomedical nanotechnology
Volume7
Issue number1
DOIs
StatePublished - Jan 1 2011
Externally publishedYes

Keywords

  • Dynamic light scattering
  • Flow cytometry
  • Propylene glycol
  • TiO nanoparticles

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

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