Stalk mechanism of vesicle fusion - Intermixing of aqueous contents

M. M. Kozlov, S. L. Leikin, L. V. Chernomordik, V. S. Markin, Y. A. Chizmadzhev

Research output: Contribution to journalArticle

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Abstract

Å mechanism for rupture of a separating bilayer, resulting from vesicle monolayer fusion is investigated theoretically. The stalk mechanism of monolayer fusion, assuming the formation and expansion of a stalk between two interacting membranes is considered. The stalk evolution leads to formation of a separating bilayer and mechanical tension appearance in the system. This tension results in rupture of the separating bilayer and hydrophilic pore formation. Competition between the mechanical tension and hydrophilic pore energy defines the criteria of contacting bilayer rupture. The tension increases with an increase of the absolute value of the negative spontaneous curvature of the outer membrane monolayer, Ks o. The pore edge energy decreases with an increase of the positive spontaneous curvature of the inner membrane monolayer, Ks i. The relations of spontaneous curvatures of outer and inner monolayers, leading to separating bilayer rupture, is calculated. It is demonstrated that his process is possible, provided spontaneous curvatures of membrane monolayers have opposite signs: Ks o<0, Ks i<0. Experimental data concerning the fusion process are analysed.

Original languageEnglish (US)
Pages (from-to)121-129
Number of pages9
JournalEuropean Biophysics Journal
Volume17
Issue number3
DOIs
StatePublished - Sep 1989

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Rupture
Membranes
Unilamellar Liposomes

Keywords

  • monolayer fusion
  • pore formation
  • stalk evolution
  • stalk mechanism
  • trilaminar structure
  • Vesicle fusion

ASJC Scopus subject areas

  • Biophysics

Cite this

Kozlov, M. M., Leikin, S. L., Chernomordik, L. V., Markin, V. S., & Chizmadzhev, Y. A. (1989). Stalk mechanism of vesicle fusion - Intermixing of aqueous contents. European Biophysics Journal, 17(3), 121-129. https://doi.org/10.1007/BF00254765

Stalk mechanism of vesicle fusion - Intermixing of aqueous contents. / Kozlov, M. M.; Leikin, S. L.; Chernomordik, L. V.; Markin, V. S.; Chizmadzhev, Y. A.

In: European Biophysics Journal, Vol. 17, No. 3, 09.1989, p. 121-129.

Research output: Contribution to journalArticle

Kozlov, MM, Leikin, SL, Chernomordik, LV, Markin, VS & Chizmadzhev, YA 1989, 'Stalk mechanism of vesicle fusion - Intermixing of aqueous contents', European Biophysics Journal, vol. 17, no. 3, pp. 121-129. https://doi.org/10.1007/BF00254765
Kozlov MM, Leikin SL, Chernomordik LV, Markin VS, Chizmadzhev YA. Stalk mechanism of vesicle fusion - Intermixing of aqueous contents. European Biophysics Journal. 1989 Sep;17(3):121-129. https://doi.org/10.1007/BF00254765
Kozlov, M. M. ; Leikin, S. L. ; Chernomordik, L. V. ; Markin, V. S. ; Chizmadzhev, Y. A. / Stalk mechanism of vesicle fusion - Intermixing of aqueous contents. In: European Biophysics Journal. 1989 ; Vol. 17, No. 3. pp. 121-129.
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