Standard operating procedures for biospecimen collection, processing, and storage: From the consortium for the study of chronic pancreatitis, diabetes, and pancreatic cancer

William E. Fisher, Zobeida Cruz-Monserrate, Amy L. McElhany, Gregory B. Lesinski, Phil A. Hart, Ria Ghosh, George Van Buren, Douglas S. Fishman, Jo Ann S. Rinaudo, Jose Serrano, Sudhir Srivastava, Thomas MacE, Mark Topazian, Ziding Feng, Dhiraj Yadav, Stephen J. Pandol, Steven J. Hughes, Robert Y. Liu, Emily Lu, Robert OrrDavid C. Whitcomb, Amer S. Abouhamze, Hanno Steen, Zachary M. Sellers, David M Troendle, Aliye Uc, Mark E. Lowe, Darwin L. Conwell

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

High-quality and well-annotated biorepositories are needed to better understand the pathophysiology and biologic mechanisms of chronic pancreatitis (CP) and its consequences. We report a methodology for the development of a robust standard operating procedure (SOP) for a biorepository based on the experience of the clinical centers within the consortium to study Chronic Pancreatitis, Diabetes and Pancreas Cancer Clinical Centers (CPDPC), supported by the National Cancer Institute and the National Institute for Diabetes and Digestive and Kidney Diseases as a unique multidisciplinary model to study CP, diabetes, and pancreatic cancer in both children and adults. Standard operating procedures from the CPDPC centers were evaluated and consolidated. The literature was reviewed for standard biorepository operating procedures that facilitated downstream molecular analysis. The existing literature on biobanking practices was harmonized with the SOPs from the clinical centers to produce a biorepository for pancreatic research. This article reports the methods and basic principles behind the creation of SOPs to develop a biorepository for the CPDPC. These will serve as a guide for investigators developing biorepositories in pancreas research. Rigorous and meticulous adherence to standardized biospecimen collection will facilitate investigations to better understand the pathophysiology and biologic mechanisms of CP, diabetes, and pancreatic cancer.

Original languageEnglish (US)
Pages (from-to)1213-1221
Number of pages9
JournalPancreas
Volume47
Issue number10
DOIs
StatePublished - Nov 1 2018
Externally publishedYes

Keywords

  • biorepository
  • biospecimens
  • pancreas
  • standard operating procedures

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

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  • Cite this

    Fisher, W. E., Cruz-Monserrate, Z., McElhany, A. L., Lesinski, G. B., Hart, P. A., Ghosh, R., Van Buren, G., Fishman, D. S., Rinaudo, J. A. S., Serrano, J., Srivastava, S., MacE, T., Topazian, M., Feng, Z., Yadav, D., Pandol, S. J., Hughes, S. J., Liu, R. Y., Lu, E., ... Conwell, D. L. (2018). Standard operating procedures for biospecimen collection, processing, and storage: From the consortium for the study of chronic pancreatitis, diabetes, and pancreatic cancer. Pancreas, 47(10), 1213-1221. https://doi.org/10.1097/MPA.0000000000001171