Starch binding domain-containing protein 1/genethonin 1 is a novel participant in glycogen metabolism

Sixin Jiang, Brigitte Heller, Vincent S. Tagliabracci, Lanmin Zhai, Jose M. Irimia, Anna A. DePaoli-Roach, Clark D. Wells, Alexander V. Skurat, Peter J. Roach

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Stbd1 is a protein of previously unknown function that is most prevalent in liver and muscle, the major sites for storage of the energy reserve glycogen. The protein is predicted to contain a hydrophobic N terminus and a C-terminal CBM20 glycan binding domain. Here, we show that Stbd1 binds to glycogen in vitro and that endogenous Stbd1 locates to perinuclear compartments in cultured mouse FL83B or Rat1 cells. When overexpressed in COSM9 cells, Stbd1 concentrated at enlarged perinuclear structures, co-localized with glycogen, the late endosomal/lysosomal marker LAMP1 and the autophagy protein GABARAPL1. Mutant Stbd1 lacking the N-terminal hydrophobic segment had a diffuse distribution throughout the cell. Point mutations in the CBM20 domain did not change the perinuclear localization of Stbd1, but glycogen was no longer concentrated in this compartment. Stable overexpression of glycogen synthase in Rat1WT4 cells resulted in accumulation of glycogen as massive perinuclear deposits, where a large fraction of the detectable Stbd1 co-localized. Starvation of Rat1WT4 cells for glucose resulted in dissipation of the massive glycogen stores into numerous and much smaller glycogen deposits that retained Stbd1. In vitro, in cells, and in animal models, Stbd1 consistently tracked with glycogen. We conclude that Stbd1 is involved in glycogen metabolism by binding to glycogen and anchoring it to membranes, thereby affecting its cellular localization and its intracellular trafficking to lysosomes.

Original languageEnglish (US)
Pages (from-to)34960-34971
Number of pages12
JournalJournal of Biological Chemistry
Volume285
Issue number45
DOIs
StatePublished - Nov 5 2010

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Glycogen
Metabolism
Starch
Proteins
Lysosomal-Associated Membrane Protein 1
Deposits
Protein Domains
Glycogen Synthase
Autophagy
Starvation
Lysosomes
Point Mutation
Liver
Polysaccharides
Muscle
Animals
Animal Models
Membranes
Glucose
Muscles

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Starch binding domain-containing protein 1/genethonin 1 is a novel participant in glycogen metabolism. / Jiang, Sixin; Heller, Brigitte; Tagliabracci, Vincent S.; Zhai, Lanmin; Irimia, Jose M.; DePaoli-Roach, Anna A.; Wells, Clark D.; Skurat, Alexander V.; Roach, Peter J.

In: Journal of Biological Chemistry, Vol. 285, No. 45, 05.11.2010, p. 34960-34971.

Research output: Contribution to journalArticle

Jiang, S, Heller, B, Tagliabracci, VS, Zhai, L, Irimia, JM, DePaoli-Roach, AA, Wells, CD, Skurat, AV & Roach, PJ 2010, 'Starch binding domain-containing protein 1/genethonin 1 is a novel participant in glycogen metabolism', Journal of Biological Chemistry, vol. 285, no. 45, pp. 34960-34971. https://doi.org/10.1074/jbc.M110.150839
Jiang, Sixin ; Heller, Brigitte ; Tagliabracci, Vincent S. ; Zhai, Lanmin ; Irimia, Jose M. ; DePaoli-Roach, Anna A. ; Wells, Clark D. ; Skurat, Alexander V. ; Roach, Peter J. / Starch binding domain-containing protein 1/genethonin 1 is a novel participant in glycogen metabolism. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 45. pp. 34960-34971.
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