STAT2 dependent Type I Interferon response promotes dysbiosis and luminal expansion of the enteric pathogen Salmonella Typhimurium

R. Paul Wilson, Sarah A. Tursi, Glenn J. Rapsinski, Nicole J. Medeiros, Long S. Le, Kevin P. Kotredes, Sajan Patel, Elisabetta Liverani, Shuang Sun, Wenhan Zhu, Laurie Kilpatrick, Sebastian E Winter, Ana M. Gamero, Çagla Tükel

Research output: Contribution to journalArticle

Abstract

The mechanisms by which the gut luminal environment is disturbed by the immune system to foster pathogenic bacterial growth and survival remain incompletely understood. Here, we show that STAT2 dependent type I IFN signaling contributes to the inflammatory environment by disrupting hypoxia enabling the pathogenic S. Typhimurium to outgrow the microbiota. Stat2-/- mice infected with S. Typhimurium exhibited impaired type I IFN induced transcriptional responses in cecal tissue and reduced bacterial burden in the intestinal lumen compared to infected wild-type mice. Although inflammatory pathology was similar between wild-type and Stat2-/- mice, we observed decreased hypoxia in the gut tissue of Stat2-/- mice. Neutrophil numbers were similar in wild-type and Stat2-/- mice, yet Stat2-/- mice showed reduced levels of myeloperoxidase activity. In vitro, the neutrophils from Stat2-/- mice produced lower levels of superoxide anion upon stimulation with the bacterial ligand N-formylmethionyl-leucyl-phenylalanine (fMLP) in the presence of IFNα compared to neutrophils from wild-type mice, indicating that the neutrophils were less functional in Stat2-/- mice. Cytochrome bd-II oxidase-mediated respiration enhances S. Typhimurium fitness in wild-type mice, while in Stat2-/- deficiency, this respiratory pathway did not provide a fitness advantage. Furthermore, luminal expansion of S. Typhimurium in wild-type mice was blunted in Stat2-/- mice. Compared to wild-type mice which exhibited a significant perturbation in Bacteroidetes abundance, Stat2-/- mice exhibited significantly less perturbation and higher levels of Bacteroidetes upon S. Typhimurium infection. Our results highlight STAT2 dependent type I IFN mediated inflammation in the gut as a novel mechanism promoting luminal expansion of S. Typhimurium.

Original languageEnglish (US)
Pages (from-to)e1007745
JournalPLoS pathogens
Volume15
Issue number4
DOIs
StatePublished - Apr 1 2019

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Dysbiosis
Interferon Type I
Salmonella typhimurium
Neutrophils
Bacteroidetes
N-Formylmethionine Leucyl-Phenylalanine
Salmonella Infections
Microbiota
Cytochromes

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Molecular Biology
  • Genetics
  • Virology

Cite this

Wilson, R. P., Tursi, S. A., Rapsinski, G. J., Medeiros, N. J., Le, L. S., Kotredes, K. P., ... Tükel, Ç. (2019). STAT2 dependent Type I Interferon response promotes dysbiosis and luminal expansion of the enteric pathogen Salmonella Typhimurium. PLoS pathogens, 15(4), e1007745. https://doi.org/10.1371/journal.ppat.1007745

STAT2 dependent Type I Interferon response promotes dysbiosis and luminal expansion of the enteric pathogen Salmonella Typhimurium. / Wilson, R. Paul; Tursi, Sarah A.; Rapsinski, Glenn J.; Medeiros, Nicole J.; Le, Long S.; Kotredes, Kevin P.; Patel, Sajan; Liverani, Elisabetta; Sun, Shuang; Zhu, Wenhan; Kilpatrick, Laurie; Winter, Sebastian E; Gamero, Ana M.; Tükel, Çagla.

In: PLoS pathogens, Vol. 15, No. 4, 01.04.2019, p. e1007745.

Research output: Contribution to journalArticle

Wilson, RP, Tursi, SA, Rapsinski, GJ, Medeiros, NJ, Le, LS, Kotredes, KP, Patel, S, Liverani, E, Sun, S, Zhu, W, Kilpatrick, L, Winter, SE, Gamero, AM & Tükel, Ç 2019, 'STAT2 dependent Type I Interferon response promotes dysbiosis and luminal expansion of the enteric pathogen Salmonella Typhimurium', PLoS pathogens, vol. 15, no. 4, pp. e1007745. https://doi.org/10.1371/journal.ppat.1007745
Wilson, R. Paul ; Tursi, Sarah A. ; Rapsinski, Glenn J. ; Medeiros, Nicole J. ; Le, Long S. ; Kotredes, Kevin P. ; Patel, Sajan ; Liverani, Elisabetta ; Sun, Shuang ; Zhu, Wenhan ; Kilpatrick, Laurie ; Winter, Sebastian E ; Gamero, Ana M. ; Tükel, Çagla. / STAT2 dependent Type I Interferon response promotes dysbiosis and luminal expansion of the enteric pathogen Salmonella Typhimurium. In: PLoS pathogens. 2019 ; Vol. 15, No. 4. pp. e1007745.
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abstract = "The mechanisms by which the gut luminal environment is disturbed by the immune system to foster pathogenic bacterial growth and survival remain incompletely understood. Here, we show that STAT2 dependent type I IFN signaling contributes to the inflammatory environment by disrupting hypoxia enabling the pathogenic S. Typhimurium to outgrow the microbiota. Stat2-/- mice infected with S. Typhimurium exhibited impaired type I IFN induced transcriptional responses in cecal tissue and reduced bacterial burden in the intestinal lumen compared to infected wild-type mice. Although inflammatory pathology was similar between wild-type and Stat2-/- mice, we observed decreased hypoxia in the gut tissue of Stat2-/- mice. Neutrophil numbers were similar in wild-type and Stat2-/- mice, yet Stat2-/- mice showed reduced levels of myeloperoxidase activity. In vitro, the neutrophils from Stat2-/- mice produced lower levels of superoxide anion upon stimulation with the bacterial ligand N-formylmethionyl-leucyl-phenylalanine (fMLP) in the presence of IFNα compared to neutrophils from wild-type mice, indicating that the neutrophils were less functional in Stat2-/- mice. Cytochrome bd-II oxidase-mediated respiration enhances S. Typhimurium fitness in wild-type mice, while in Stat2-/- deficiency, this respiratory pathway did not provide a fitness advantage. Furthermore, luminal expansion of S. Typhimurium in wild-type mice was blunted in Stat2-/- mice. Compared to wild-type mice which exhibited a significant perturbation in Bacteroidetes abundance, Stat2-/- mice exhibited significantly less perturbation and higher levels of Bacteroidetes upon S. Typhimurium infection. Our results highlight STAT2 dependent type I IFN mediated inflammation in the gut as a novel mechanism promoting luminal expansion of S. Typhimurium.",
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