Estudo estereológico das células de Purkinje cerebelares submetidas à intoxicação alcoólica em ratos Wistar

Mara Ibis Rodrigues Apfel; Charles Alfred Ésberard; Fabrício Kafury Pereira Rodrigues; Fayez Mohamad Bahamad; Rafael Oliveira Sillero

doi:10.1590/s0004-282x2002000200014

Estudo estereológico das células de Purkinje cerebelares submetidas à intoxicação alcoólica em ratos Wistar

Translated title of the contribution: Stereologic study of the cerebellar Purkinje cells submitted to alcoholic intoxication in Wistar rats

Mara Ibis Rodrigues Apfel, Charles Alfred Ésberard, Fabrício Kafury Pereira Rodrigues, Fayez Mohamad Bahamad, Rafael Oliveira Sillero

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Background: to analyze the effect of the alcohol on the cells of Purkinje. Method: Wistar rats received alcoholic solutions orally in different concentrations 4%, 12% and 24%. The animals were sacrificed with 4, 8 and 12 weeks and the cerebella were randomly cut and embedded in paraffin. Sections of 6μm (H&E) were stereologically analyzed. Results: The differences among the density for area and density of surface of the cells of Purkinje of all of the experimental groups (E) and the respective controls (C) were significant. With 12 weeks the cell of Purkinje volume density decreased among the groups C and E in the concentrations of 4% and 12%, but not for the concentration of 24%, probably due to smaller liquid ingestion by the animals. Conclusion: The alcohol has toxic effect on the Purkinje cellular body in the three studied concentrations from 4 weeks.

Translated title of the contribution	Stereologic study of the cerebellar Purkinje cells submitted to alcoholic intoxication in Wistar rats
Original language	Portuguese
Pages (from-to)	258-263
Number of pages	6
Journal	Arquivos de Neuro-Psiquiatria
Volume	60
Issue number	2 A
DOIs	https://doi.org/10.1590/s0004-282x2002000200014
State	Published - Jun 2002
Externally published	Yes

Keywords

Alcohol
Cerebellum
Purkinje cell
Stereology

ASJC Scopus subject areas

Clinical Neurology
Neurology
Biological Psychiatry

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10.1590/s0004-282x2002000200014

Cite this

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title = "Estudo estereol{\'o}gico das c{\'e}lulas de Purkinje cerebelares submetidas {\`a} intoxica{\c c}{\~a}o alco{\'o}lica em ratos Wistar",

abstract = "Background: to analyze the effect of the alcohol on the cells of Purkinje. Method: Wistar rats received alcoholic solutions orally in different concentrations 4%, 12% and 24%. The animals were sacrificed with 4, 8 and 12 weeks and the cerebella were randomly cut and embedded in paraffin. Sections of 6μm (H&E) were stereologically analyzed. Results: The differences among the density for area and density of surface of the cells of Purkinje of all of the experimental groups (E) and the respective controls (C) were significant. With 12 weeks the cell of Purkinje volume density decreased among the groups C and E in the concentrations of 4% and 12%, but not for the concentration of 24%, probably due to smaller liquid ingestion by the animals. Conclusion: The alcohol has toxic effect on the Purkinje cellular body in the three studied concentrations from 4 weeks.",

keywords = "Alcohol, Cerebellum, Purkinje cell, Stereology",

author = "Apfel, {Mara Ibis Rodrigues} and {\'E}sberard, {Charles Alfred} and Rodrigues, {Fabr{\'i}cio Kafury Pereira} and Bahamad, {Fayez Mohamad} and Sillero, {Rafael Oliveira}",

year = "2002",

month = jun,

doi = "10.1590/s0004-282x2002000200014",

language = "Portuguese",

volume = "60",

pages = "258--263",

journal = "Arquivos de Neuro-Psiquiatria",

issn = "0004-282X",

publisher = "Arquivos de Neuro-Psiquiatria",

number = "2 A",

}

TY - JOUR

T1 - Estudo estereológico das células de Purkinje cerebelares submetidas à intoxicação alcoólica em ratos Wistar

AU - Apfel, Mara Ibis Rodrigues

AU - Ésberard, Charles Alfred

AU - Rodrigues, Fabrício Kafury Pereira

AU - Bahamad, Fayez Mohamad

AU - Sillero, Rafael Oliveira

PY - 2002/6

Y1 - 2002/6

N2 - Background: to analyze the effect of the alcohol on the cells of Purkinje. Method: Wistar rats received alcoholic solutions orally in different concentrations 4%, 12% and 24%. The animals were sacrificed with 4, 8 and 12 weeks and the cerebella were randomly cut and embedded in paraffin. Sections of 6μm (H&E) were stereologically analyzed. Results: The differences among the density for area and density of surface of the cells of Purkinje of all of the experimental groups (E) and the respective controls (C) were significant. With 12 weeks the cell of Purkinje volume density decreased among the groups C and E in the concentrations of 4% and 12%, but not for the concentration of 24%, probably due to smaller liquid ingestion by the animals. Conclusion: The alcohol has toxic effect on the Purkinje cellular body in the three studied concentrations from 4 weeks.

AB - Background: to analyze the effect of the alcohol on the cells of Purkinje. Method: Wistar rats received alcoholic solutions orally in different concentrations 4%, 12% and 24%. The animals were sacrificed with 4, 8 and 12 weeks and the cerebella were randomly cut and embedded in paraffin. Sections of 6μm (H&E) were stereologically analyzed. Results: The differences among the density for area and density of surface of the cells of Purkinje of all of the experimental groups (E) and the respective controls (C) were significant. With 12 weeks the cell of Purkinje volume density decreased among the groups C and E in the concentrations of 4% and 12%, but not for the concentration of 24%, probably due to smaller liquid ingestion by the animals. Conclusion: The alcohol has toxic effect on the Purkinje cellular body in the three studied concentrations from 4 weeks.

KW - Alcohol

KW - Cerebellum

KW - Purkinje cell

KW - Stereology

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SN - 0004-282X

VL - 60

SP - 258

EP - 263

JO - Arquivos de Neuro-Psiquiatria

JF - Arquivos de Neuro-Psiquiatria

IS - 2 A

ER -

Estudo estereológico das células de Purkinje cerebelares submetidas à intoxicação alcoólica em ratos Wistar

Abstract

Keywords

ASJC Scopus subject areas

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