Steroid 5α-reductase 1 promotes 5α-androstane-3α, 17β-diol synthesis in immature mouse testes by two pathways

Mala Mahendroo, Jean D. Wilson, James A. Richardson, Richard J. Auchus

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

5α-Androstane-3α,17β-diol (androstanediol) is the predominant androgen in immature mouse testes, and studies were designed to investigate its pathway of synthesis, the steroid 5α-reductase isoenzyme involved in its formation, and whether testicular androstanediol is formed in embryonic mouse testes at the time of male phenotypic development. In 24-26-day-old immature testes, androstanediol is formed by two pathways; the predominant one involves testosterone → dihydrotestosterone → androstanediol, and a second utilizes the pathway progesterone → 5α-dihydroprogesterone → 5α-pregnane-3α-ol-20-one → 5α-pregnane-3α,17α-diol-20-one → androsterone → androstanediol. Formation of androstanediol was normal in testes from mice deficient in steroid 5α-reductase 2 but absent in testes from mice deficient in steroid 5α-reductase 1, indicating that isoenzyme 2 is not expressed in day 24-26 testes. The fact that androstenedione and testosterone were the only androgens identified after incubation of day 16 and 17 embryonic testes with [3H]progesterone implies that androstanediol formation in the testis plays no role in male phenotypic differentiation in the mouse.

Original languageEnglish (US)
Pages (from-to)113-120
Number of pages8
JournalMolecular and Cellular Endocrinology
Volume222
Issue number1-2
DOIs
StatePublished - Jul 30 2004

Keywords

  • 17β-(N,N-diethyl)carbamoyl-4-methyl-4-aza-5α-androstan-3-one
  • 4MA
  • 5α-androstane-3α,17β-diol
  • Androstanediol
  • HPLC
  • Mouse testes
  • Neonatal androgen
  • Steroid 5α-reductase
  • high-performance liquid chromatography

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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