Sterol-regulated ubiquitination and degradation of Insig-1 creates a convergent mechanism for feedback control of cholesterol synthesis and uptake

Yi Gong, Joon No Lee, Peter C W Lee, Joseph L. Goldstein, Michael S. Brown, Jin Ye

Research output: Contribution to journalArticle

158 Scopus citations

Abstract

This paper describes a convergent mechanism for the feedback control of cholesterol synthesis and uptake mediated by SREBPs, membrane bound transcription factors. Endoplasmic reticulum (ER) bound SREBPs form complexes with Scap, a polytopic ER protein. In sterol-overloaded cells, Scap/SREBP binds to Insig-1, which retains the complex in the ER. Upon sterol deprivation, the Scap/SREBP complex dissociates from Insig-1, which is then ubiquitinated on lysines 156 and 158 and degraded in proteasomes. Scap/SREBP moves to the Golgi, where SREBP is processed to liberate a nuclear fragment that activates genes for cholesterol synthesis and uptake and the gene for Insig-1. Ubiquitination is not necessary for release of Scap/SREBP from Insig-1, but it establishes a requirement for synthesis of new Insig-1 for feedback inhibition. When the new Insig-1 and cholesterol converge on Scap, Scap/SREBP binds to Insig-1, preventing ubiquitination. The Insig-1/Scap/SREBP complex accumulates in the ER, ready for liberation when the cell is again sterol deprived.

Original languageEnglish (US)
Pages (from-to)15-24
Number of pages10
JournalCell Metabolism
Volume3
Issue number1
DOIs
StatePublished - Jan 1 2006

Keywords

  • Humdisease
  • Proteins

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

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