Cultured cells that respond to adenosine and 2 chloroadenosine with stimulation of accumulation of cyclic AMP were incubated with 3H 2 chloroadenosine. Cyclic AMP synthesis was stimulated with isoproterenol or 2 chloroadenosine, and the possible incorporation of 3H into cyclic AMP or 2 chloro cyclic AMP was evaluated. Such incorporation was not found to be significant. There is thus no evidence that 2 chloroadenosine and related nucleosides stimulate cyclic AMP synthesis by increasing the availability of substrate for adenylate cyclase.
|Original language||English (US)|
|Title of host publication||J.CYCLIC NUCLEOTIDE RES.|
|Number of pages||10|
|State||Published - 1975|
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