Stimulation of growth hormone synthesis by glucose in islets of langerhans isolated from transgenic mice

M. Welsh, Robert E Hammer, R. L. Brinster, D. F. Steiner

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Abstract

To examine further the mechanism by which the synthesis of proteins translated on the rough endoplasmic reticulum is regulated in pancreatic β-cells, the synthesis of growth hormone in islets from transgenic mice carrying the metallothionein-rat growth hormone gene fusion was studied. High glucose (17 mM) stimulated the synthesis and secretion of an apparently normally processed growth hormone. The stimulation of synthesis of growth hormone was less efficient than the stimulation of insulin synthesis in these islets, whereas the stimulation of release of labeled growth hormone paralleled that of insulin. These relults are consistent with the hypothesis that signal recognition particle-mediated mechanism(s) may be involved in regulating the translational efficiency of secreted proteins in isolated islets (Welsh, M., Scherberg, N., Gilmore, R., and Steiner, D.F. (1986) Biochem. J. 235, 459-467). Furthermore, in the β-cells, growth hormone follows the normal regulated pathway of secretory granule transport and exocytosis.

Original languageEnglish (US)
Pages (from-to)12915-12917
Number of pages3
JournalJournal of Biological Chemistry
Volume261
Issue number28
StatePublished - 1986

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Islets of Langerhans
Transgenic Mice
Growth Hormone
Glucose
Signal Recognition Particle
Insulin
Rough Endoplasmic Reticulum
Metallothionein
Gene Fusion
Exocytosis
Secretory Vesicles
Cell growth
Rats
Proteins
Fusion reactions
Genes

ASJC Scopus subject areas

  • Biochemistry

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Stimulation of growth hormone synthesis by glucose in islets of langerhans isolated from transgenic mice. / Welsh, M.; Hammer, Robert E; Brinster, R. L.; Steiner, D. F.

In: Journal of Biological Chemistry, Vol. 261, No. 28, 1986, p. 12915-12917.

Research output: Contribution to journalArticle

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