Stimulation of growth hormone synthesis by glucose in islets of langerhans isolated from transgenic mice

M. Welsh, Robert E Hammer, R. L. Brinster, D. F. Steiner

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

To examine further the mechanism by which the synthesis of proteins translated on the rough endoplasmic reticulum is regulated in pancreatic β-cells, the synthesis of growth hormone in islets from transgenic mice carrying the metallothionein-rat growth hormone gene fusion was studied. High glucose (17 mM) stimulated the synthesis and secretion of an apparently normally processed growth hormone. The stimulation of synthesis of growth hormone was less efficient than the stimulation of insulin synthesis in these islets, whereas the stimulation of release of labeled growth hormone paralleled that of insulin. These relults are consistent with the hypothesis that signal recognition particle-mediated mechanism(s) may be involved in regulating the translational efficiency of secreted proteins in isolated islets (Welsh, M., Scherberg, N., Gilmore, R., and Steiner, D.F. (1986) Biochem. J. 235, 459-467). Furthermore, in the β-cells, growth hormone follows the normal regulated pathway of secretory granule transport and exocytosis.

Original languageEnglish (US)
Pages (from-to)12915-12917
Number of pages3
JournalJournal of Biological Chemistry
Volume261
Issue number28
StatePublished - Dec 1 1986

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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