Stimulation of MAP kinase pathways after maternal IL-1β exposure induces fetal lung fluid absorption in guinea pigs

Reshma Bhattacharjee, Tianbo Li, Shyny Koshy, LaMonta L. Beard, Kapil Sharma, Ethan P. Carter, Chrystelle Garat, Hans G. Folkesson

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: We tested the hypothesis that maternal interleukin-1β (IL-1β) pretreatment and induction of fetal cortisol synthesis activates MAP kinases and thereby affects lung fluid absorption in preterm guinea pigs.Methods: IL-1β was administered subcutaneously daily to timed-pregnant guinea pigs for three days. Fetuses were obtained by abdominal hysterotomy and instilled with isosmolar 5% albumin into the lungs and lung fluid movement was measured over 1 h by mass balance. MAP kinase expression was measured by western blot.Results: Lung fluid absorption was induced at 61 days (D) gestation and stimulated at 68D gestation by IL-1β. Maternal IL-1β pretreatment upregulated ERK and upstream MEK expression at both 61 and 68D gestation, albeit being much more pronounced at 61D gestation. U0126 instillation completely blocked IL-1β-induced lung fluid absorption as well as IL-1β-induced/stimulated ERK expression. Cortisol synthesis inhibition by metyrapone attenuated ERK expression and lung fluid absorption in IL-1β-pretreated fetal lungs. JNK expression after maternal IL-1β pretreatment remained unaffected at either gestation age.Conclusion: These data implicate the ERK MAP kinase pathway as being important for IL-1β induction/stimulation of lung fluid absorption in fetal guinea pigs.

Original languageEnglish (US)
Article number27
JournalRespiratory Research
Volume8
DOIs
StatePublished - Mar 26 2007

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Interleukin-1
Guinea Pigs
Phosphotransferases
Mothers
Lung
Pregnancy
Hydrocortisone
Hysterotomy
Metyrapone
Mitogen-Activated Protein Kinase Kinases
Extracellular Signal-Regulated MAP Kinases
Albumins
Fetus
Western Blotting

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Stimulation of MAP kinase pathways after maternal IL-1β exposure induces fetal lung fluid absorption in guinea pigs. / Bhattacharjee, Reshma; Li, Tianbo; Koshy, Shyny; Beard, LaMonta L.; Sharma, Kapil; Carter, Ethan P.; Garat, Chrystelle; Folkesson, Hans G.

In: Respiratory Research, Vol. 8, 27, 26.03.2007.

Research output: Contribution to journalArticle

Bhattacharjee, Reshma ; Li, Tianbo ; Koshy, Shyny ; Beard, LaMonta L. ; Sharma, Kapil ; Carter, Ethan P. ; Garat, Chrystelle ; Folkesson, Hans G. / Stimulation of MAP kinase pathways after maternal IL-1β exposure induces fetal lung fluid absorption in guinea pigs. In: Respiratory Research. 2007 ; Vol. 8.
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abstract = "Background: We tested the hypothesis that maternal interleukin-1β (IL-1β) pretreatment and induction of fetal cortisol synthesis activates MAP kinases and thereby affects lung fluid absorption in preterm guinea pigs.Methods: IL-1β was administered subcutaneously daily to timed-pregnant guinea pigs for three days. Fetuses were obtained by abdominal hysterotomy and instilled with isosmolar 5{\%} albumin into the lungs and lung fluid movement was measured over 1 h by mass balance. MAP kinase expression was measured by western blot.Results: Lung fluid absorption was induced at 61 days (D) gestation and stimulated at 68D gestation by IL-1β. Maternal IL-1β pretreatment upregulated ERK and upstream MEK expression at both 61 and 68D gestation, albeit being much more pronounced at 61D gestation. U0126 instillation completely blocked IL-1β-induced lung fluid absorption as well as IL-1β-induced/stimulated ERK expression. Cortisol synthesis inhibition by metyrapone attenuated ERK expression and lung fluid absorption in IL-1β-pretreated fetal lungs. JNK expression after maternal IL-1β pretreatment remained unaffected at either gestation age.Conclusion: These data implicate the ERK MAP kinase pathway as being important for IL-1β induction/stimulation of lung fluid absorption in fetal guinea pigs.",
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AU - Bhattacharjee, Reshma

AU - Li, Tianbo

AU - Koshy, Shyny

AU - Beard, LaMonta L.

AU - Sharma, Kapil

AU - Carter, Ethan P.

AU - Garat, Chrystelle

AU - Folkesson, Hans G.

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