TY - JOUR
T1 - Stimulus/secretion coupling factors in glucose-stimulated insulin secretion
T2 - Insights gained from a multidisciplinary approach
AU - Newgard, Christopher B.
AU - Lu, Danhong
AU - Jensen, Mette Valentin
AU - Schissler, Jonathan
AU - Boucher, Anne
AU - Burgess, Shawn
AU - Sherry, A. Dean
PY - 2002/12/1
Y1 - 2002/12/1
N2 - There is a growing appreciation for the complexity of the pathways involved in glucose-stimulated insulin secretion (GSIS) from pancreatic islet β-cells. In our laboratory, this has stimulated the development of an interdisciplinary approach to the problem. In this study, we review recent studies combining the tools of recombinant adenovirus for gene delivery, the development of novel cell lines that exhibit either robust or weak GSIS, and nuclear magnetic resonance imaging for metabolic fingerprinting of glucose-stimulated cells. Using these tools, we demonstrate a potentially important role for pyruvate carboxylase -mediated pyruvate cycling pathways in the control of GSIS, and discuss potential coupling factors produced by such pathways.
AB - There is a growing appreciation for the complexity of the pathways involved in glucose-stimulated insulin secretion (GSIS) from pancreatic islet β-cells. In our laboratory, this has stimulated the development of an interdisciplinary approach to the problem. In this study, we review recent studies combining the tools of recombinant adenovirus for gene delivery, the development of novel cell lines that exhibit either robust or weak GSIS, and nuclear magnetic resonance imaging for metabolic fingerprinting of glucose-stimulated cells. Using these tools, we demonstrate a potentially important role for pyruvate carboxylase -mediated pyruvate cycling pathways in the control of GSIS, and discuss potential coupling factors produced by such pathways.
UR - http://www.scopus.com/inward/record.url?scp=0036894293&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036894293&partnerID=8YFLogxK
U2 - 10.2337/diabetes.51.2007.s389
DO - 10.2337/diabetes.51.2007.s389
M3 - Article
C2 - 12475781
AN - SCOPUS:0036894293
SN - 0012-1797
VL - 51
SP - S389-S393
JO - Diabetes
JF - Diabetes
IS - SUPPL. 3
ER -