Stoichiometry of chromatin proteins

W. T. Garrard; W. R. Pearson; S. K. Wake; James Bonner

doi:10.1016/0006-291X(74)90889-4

Stoichiometry of chromatin proteins

W. T. Garrard, W. R. Pearson, S. K. Wake, James Bonner

Molecular Biology

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The stoichiometry of rat liver chromatin proteins has been estimated by quantitative disc electrophoresis. There are about 1.3 × 10⁸ histone and 2.4 × 10⁷ nonhistone polypeptide molecules per haploid genome, an average of one such molecule for every 21 and 117 base pairs of DNA, respectively. Nonhistone proteins have been separated into 115 polypeptide size classes. The total number of molecules represented in distinct size classes ranges from 4.2 × 10³ (limits of sensitivity) to 1.7 × 10⁶ per haploid genome, with a mean of 2.1 × 10⁵.

Original language	English (US)
Pages (from-to)	50-57
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	58
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(74)90889-4
State	Published - May 7 1974

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(74)90889-4

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abstract = "The stoichiometry of rat liver chromatin proteins has been estimated by quantitative disc electrophoresis. There are about 1.3 × 108 histone and 2.4 × 107 nonhistone polypeptide molecules per haploid genome, an average of one such molecule for every 21 and 117 base pairs of DNA, respectively. Nonhistone proteins have been separated into 115 polypeptide size classes. The total number of molecules represented in distinct size classes ranges from 4.2 × 103 (limits of sensitivity) to 1.7 × 106 per haploid genome, with a mean of 2.1 × 105.",

author = "Garrard, {W. T.} and Pearson, {W. R.} and Wake, {S. K.} and James Bonner",

note = "Funding Information: ACKNOWLEDGEMENTS. -- This work was supported in part vice Grant GM 13762, by Damon Runyon Memorial Fund DRF-755 (W. T. G.), by U. S. Public Health Service (W. R. P.), and by The California Foundation for",

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AU - Garrard, W. T.

AU - Pearson, W. R.

AU - Wake, S. K.

AU - Bonner, James

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N2 - The stoichiometry of rat liver chromatin proteins has been estimated by quantitative disc electrophoresis. There are about 1.3 × 108 histone and 2.4 × 107 nonhistone polypeptide molecules per haploid genome, an average of one such molecule for every 21 and 117 base pairs of DNA, respectively. Nonhistone proteins have been separated into 115 polypeptide size classes. The total number of molecules represented in distinct size classes ranges from 4.2 × 103 (limits of sensitivity) to 1.7 × 106 per haploid genome, with a mean of 2.1 × 105.

AB - The stoichiometry of rat liver chromatin proteins has been estimated by quantitative disc electrophoresis. There are about 1.3 × 108 histone and 2.4 × 107 nonhistone polypeptide molecules per haploid genome, an average of one such molecule for every 21 and 117 base pairs of DNA, respectively. Nonhistone proteins have been separated into 115 polypeptide size classes. The total number of molecules represented in distinct size classes ranges from 4.2 × 103 (limits of sensitivity) to 1.7 × 106 per haploid genome, with a mean of 2.1 × 105.

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Stoichiometry of chromatin proteins

Abstract

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